Ultra-LEAF™ Purified anti-mouse CD47 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking² and immunohistochemical staining of frozen sections³. This clone is not cross-reactive with human CD47.

Application References

(PubMed link indicates BioLegend citation)

1. Wang H, et al. 2007. P. Natl. Acad. Sci. USA 104:13744.
2. Jiang P, et al. 1999 J. Biol. Chem. 274:559. (IHC-F, Block)

Product Citations

1. Wang F, et al. 2023. Proc Natl Acad Sci U S A. e2204621120:120. PubMed
2. Morrissey MA, et al. 2020. Immunity. 53(2):290-302.e6. PubMed
3. Zhou Y, et al. 2022. Theranostics. 12:5488. PubMed

RRID

AB_2571996 (BioLegend Cat. No. 127519)
AB_2571996 (BioLegend Cat. No. 127534)
AB_2571996 (BioLegend Cat. No. 127533)
AB_2571996 (BioLegend Cat. No. 127520)
AB_2571996 (BioLegend Cat. No. 127517)
AB_2571996 (BioLegend Cat. No. 127518)

Antigen Details

Structure

50 kD plasma membrane protein of the immunoglobulin supergene family, with an extracellular IgV-like domain of 120 amino acids, a five membrane spanning segment of 152 amino acids, and a short cytoplasmic tail of 30 amino acids.

Distribution

Broad tissue distribution, including haemopoietic cells, epithelial and endothelial cells, fibroblasts, erythrocytes and other tissue cells.

Function

Adhesion molecules, immune regulation.

Interaction

Associates with β3 integrins.

Ligand/Receptor

SIRPα (CD172α, Signal regulatory protein alpha), thrombospondin.

Bioactivity

Upon interaction with SIRPα, controls innate immune effector function, such as host cell phagocytosis.

Biology Area

Immunology, Innate Immunity

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Brown E, et al. 1990. J. Cell. Biol. 111:2785.
2. Mawby WJ, et al. 1994. Biochem. J. 304:525.
3. Gao AG, et al. 1996. J. Biol. Chem. 271:21.
4. Oldenborg PA, et al. 2000. Science 288:2051.
5. van Beek EM, et al. 2005. J. Immunol. 175:7781.

Gene ID

16423 View all products for this Gene ID

UniProt

View information about CD47 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis