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Alexa Fluor® 647 anti-mouse CD199 (CCR9) Antibody

Pricing & Availability
Clone
CW-1.2 (See other available formats)
Regulatory Status
RUO
Other Names
CCR9, GPR-9-6,GPR28, CDw199
Isotype
Mouse IgG2a, κ
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Product Citations
publications
CW-1dot2_Alx647_090208.jpg
C57BL/6 thymocytes stained with CW-1.2 Alexa Fluor® 647
  • CW-1dot2_Alx647_090208.jpg
    C57BL/6 thymocytes stained with CW-1.2 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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128707 25 µg 118€
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128708 100 µg 278€
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Description

CCR9 is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in gut-associated immune response. Mouse CCR9 is highly expressed on different stages of thymocytes and upregulated on CD4+CD8+ cells. It is expressed to a much lower level on spleen and lymph node cells. Expression of CCR9 on γ/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Kawabe T, et al. 2013. J Immunol. 190:5788. PubMed
RRID
AB_1186164 (BioLegend Cat. No. 128707)
AB_1186164 (BioLegend Cat. No. 128708)

Antigen Details

Structure
A membrane of G-protein coupled receptors and contains seven transmembrain domains. Mouse CCR9 has above 80% amino acid sequence homology with human counterpart.
Distribution

CD4+CD8+ thymocytes, γ/δ T cells

Interaction
CCL25
Ligand/Receptor
CCL25
Cell Type
T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References
  1. Zaballos A, et al. 1999. J. Immunol. 162:5671.
  2. Wurbel MA, et al. 2007. J. Immunol. 178:7598.
  3. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73.
Gene ID
12769 View all products for this Gene ID
UniProt
View information about CD199 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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