Biotin anti-human TIGIT VSTM3 Antibody anti-TIGIT

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Clone: A15153G (See other available formats)
Regulatory Status: RUO
Other Names: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Isotype: Mouse IgG2a, κ
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Product Citations: publications

A15153G_Biotin_TIGIT_VSTM3_Antibody_1_052217 — Human peripheral blood leukocytes were stained with CD3 FITC and biotinylated TIGIT (top) or biotinylated mouse IgG2a, κ isotype control (bottom) followed by Streptavidin PE.

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372722

100 µg

203€

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Description

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human TIGIT.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Additional reported applications (for the relevant formats) include: Blocking¹.
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.

Watch a Scientific Poster video of this product.
RRID: AB_2687252 (BioLegend Cat. No. 372722)

Antigen Details

Structure: 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.
Distribution: Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.
Function: Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
Ligand/Receptor: CD155 (PVR), CD112 (PVRL2, NECTIN-2).
Cell Type: NK cells, T cells, Tfh, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
Molecular Family: Adhesion Molecules, Immune Checkpoint Receptors
Antigen References: 1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923.
Gene ID: 201633 View all products for this Gene ID
UniProt: View information about TIGIT on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All TIGIT (VSTM3) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human TIGIT (VSTM3)	A15153G	FC,Block
APC/Fire™ 750 anti-human TIGIT (VSTM3)	A15153G	FC
APC anti-human TIGIT (VSTM3)	A15153G	FC
PE anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 421™ anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 605™ anti-human TIGIT (VSTM3)	A15153G	FC
PE/Dazzle™ 594 anti-human TIGIT (VSTM3)	A15153G	FC
PerCP/Cyanine5.5 anti-human TIGIT (VSTM3)	A15153G	FC
PE/Cyanine7 anti-human TIGIT (VSTM3)	A15153G	FC
Ultra-LEAF™ Purified anti-human TIGIT (VSTM3)	A15153G	FC
Biotin anti-human TIGIT (VSTM3)	A15153G	FC
Alexa Fluor® 647 anti-human TIGIT (VSTM3)	A15153G	FC
TotalSeq™-A0089 anti-human TIGIT (VSTM3)	A15153G	PG
TotalSeq™-B0089 anti-human TIGIT (VSTM3)	A15153G	PG
TotalSeq™-C0089 anti-human TIGIT (VSTM3)	A15153G	PG
KIRAVIA Blue 520™ anti-human TIGIT (VSTM3)	A15153G	FC
APC/Cyanine7 anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 510™ anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 785™ anti-human TIGIT (VSTM3) Antibody	A15153G	FC
TotalSeq™-D0089 anti-human TIGIT (VSTM3)	A15153G	PG
Brilliant Violet 711™ anti-human TIGIT (VSTM3)	A15153G	FC
PE/Fire™ 640 anti-human TIGIT (VSTM3)	A15153G	FC
PE/Fire™ 810 anti-human TIGIT (VSTM3)	A15153G	FC
PE/Cyanine5 anti-human TIGIT (VSTM3)	A15153G	FC
PerCP/Fire™ 806 anti-human TIGIT (VSTM3)	A15153G	FC
PerCP/Fire™ 780 anti-human TIGIT (VSTM3)	A15153G	FC
Spark Red™ 718 anti-human TIGIT (VSTM3) (Flexi-Fluor™)	A15153G	FC
PE/Fire™ 700 anti-human TIGIT (VSTM3)	A15153G	FC

Certificate of Analysis

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Go To Top Version: 0 Revision Date: 05/23/2017

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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