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Brilliant Violet 421™ anti-mouse CD144 (VE-cadherin) Antibody

Pricing & Availability
Clone
BV13 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular endothelial-cadherin
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
BV13_BV421_CD144_Antibody_FC_100713
Mouse endothelial cells bEnd.3 were stained with CD144 (clone BV13) Brilliant Violet 421™ (filled histogram) or rat IgG1 Brilliant Violet 421™ isotype control (open histogram).
  • BV13_BV421_CD144_Antibody_FC_100713
    Mouse endothelial cells bEnd.3 were stained with CD144 (clone BV13) Brilliant Violet 421™ (filled histogram) or rat IgG1 Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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138013 50 µg 203€
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Description

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
VE-cadherin-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM5. Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy staining4.

Application References
  1. Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block)
  2. Liao F, et al. 2000. Cancer Res. 60:6805. (FC)
  3. Crosby CV, et al. 2005. Blood 105:2771. (FC)
  4. Liao F, et al. 2002. Cancer Res. 62:2567. (IF)
  5. May C, et al. 2005. Blood 105:4337. (epitope)
Product Citations
  1. Komori R, et al. 2022. Int J Mol Sci. 23: . PubMed
  2. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
  3. Liu X, et al. 2021. Nat Commun. 12:1832. PubMed
  4. Tang W, et al. 2021. Front Cell Dev Biol. 9:728057. PubMed
  5. Figueiredo CA, et al. 2022. J Neuroinflammation. 19:17. PubMed
RRID
AB_2562627 (BioLegend Cat. No. 138013)

Antigen Details

Structure
A 120 kD member of type II Cadherin family
Distribution

Endothelial cells

Function
Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand/Receptor
CD144
Cell Type
Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821.
2. Hirashima M, et al. 2009. Blood 93:1253.
3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362.
4. Matsumura K, et al. 2003. Blood 101:1367.
5. Hirashima M, et al. 2009. Blood 101:2261.
6. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.

Gene ID
12562 View all products for this Gene ID
UniProt
View information about CD144 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 01/12/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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