- Clone
- BV13 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Vascular endothelial-cadherin
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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138019 | 100 µg | 185€ | ||||
138020 | 1 mg | 595€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family. It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion. VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- VE-cadherin-Ig fusion protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
WB, ICC, Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. - Application Notes
-
Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM5. Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy staining4.
- Application References
-
- Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block)
- Liao F, et al. 2000. Cancer Res. 60:6805. (FC)
- Crosby CV, et al. 2005. Blood 105:2771. (FC)
- Liao F, et al. 2002. Cancer Res. 62:2567. (IF)
- May C, et al. 2005. Blood 105:4337. (epitope)
- RRID
-
AB_2888759 (BioLegend Cat. No. 138019)
AB_2888759 (BioLegend Cat. No. 138020)
Antigen Details
- Structure
- A 120 kD member of type II Cadherin family
- Distribution
-
Endothelial cells
- Function
- Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
- Ligand/Receptor
- CD144
- Cell Type
- Endothelial cells, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821.
2. Hirashima M, et al. 2009. Blood 93:1253.
3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362.
4. Matsumura K, et al. 2003. Blood 101:1367.
5. Hirashima M, et al. 2009. Blood 101:2261.
6. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729. - Gene ID
- 12562 View all products for this Gene ID
- UniProt
- View information about CD144 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD144 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD144 (VE-cadherin) | BV13 | FC,WB,ICC,Block |
Alexa Fluor® 647 anti-mouse CD144 (VE-cadherin) | BV13 | FC |
Biotin anti-mouse CD144 (VE-cadherin) | BV13 | FC |
PE anti-mouse CD144 (VE-cadherin) | BV13 | FC |
APC anti-mouse CD144 (VE-cadherin) | BV13 | FC |
Brilliant Violet 421™ anti-mouse CD144 (VE-cadherin) | BV13 | FC |
PE/Cyanine7 anti-mouse CD144 (VE-cadherin) | BV13 | FC |
TotalSeq™-A1036 anti-mouse CD144 (VE-cadherin) | BV13 | PG |
Ultra-LEAF™ Purified anti-mouse CD144 (VE-cadherin) Antibody | BV13 | FC,WB,ICC,Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD144 (VE-cadherin)
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Alexa Fluor® 647 anti-mouse CD144 (VE-cadherin)
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Biotin anti-mouse CD144 (VE-cadherin)
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PE anti-mouse CD144 (VE-cadherin)
-
APC anti-mouse CD144 (VE-cadherin)
-
Brilliant Violet 421™ anti-mouse CD144 (VE-cadherin)
-
PE/Cyanine7 anti-mouse CD144 (VE-cadherin)
-
TotalSeq™-A1036 anti-mouse CD144 (VE-cadherin)
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Ultra-LEAF™ Purified anti-mouse CD144 (VE-cadherin) Antibody
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