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Purified anti-mouse Keratin 6A Antibody (Previously Covance catalog# PRB-169P)

Pricing & Availability
Clone
Poly19057 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin, type II cytoskeletal 6A, K6A, CK-6A, mK6-alpha, keratin-6A, 60-kD keratin, cytokeratin-6A, keratin-6-alpha
Previously
Covance Catalog# PRB-169P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
A_Poly19057_PURE_Keratin6A_IHC_021715
Staining of Mouse Keratin 6, on mouse tongue at a 1:5000 dilution.
  • A_Poly19057_PURE_Keratin6A_IHC_021715
    Staining of Mouse Keratin 6, on mouse tongue at a 1:5000 dilution.
  • B_Poly19057_PURE_Keratin6A_WB_050118
    Total lysates (15 µg protein) from 293E transfected with or without mouse keratin 6A were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (1 ug/ml) purified anti-mouse keratin 6A antibody (clone Poly19057). Proteins were visualized using chemiluminescence detection by incubation with and HRP goat anti-mouse IgG secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • C_Poly19057_PURE_Keratin6A_IHC_2_031521
    IHC staining of purified anti-mouse Keratin 6A antibody (Poly19057) on formalin-fixed paraffin-embedded mouse esophagus tissue. Following antigen retrieval using Citrate (Cat. No. 420901), the tissue was incubated with 1.0 µg/mL of the primary antibody 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, AEC, Cat. No. 929501) was used for detection followed by hemotoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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905702 25 µL 72€
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905701 100 µL 231€
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Description

Keratin 6A is a type II cytokeratin that belongs to the keratin family of fibrous structural proteins. The protein is expressed along with its partners KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. The murine genome is known to have two keratin 6 (CK6) genes, CK6A and CK6b. Mutations in these genes have been associated with pachyonychia congenita.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 6 protein.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000*
• IF: 1:500
• IHC: 1:500

Application Notes

This antibody is effective in immunoblotting (WB), immunofluorescence (IF) and immunohistochemistry (IHC). This antibody has been successfully used in both frozen and paraffin embedded tissues.

*Predicted MW = 59.9 kD


This product may contain other non-IgG subtypes.

Application References
  1. Welm AL, et al. 2005. Proc Natl Acad Sci USA. 102:4324.
  2. Hu Y, et al. 2001. Nature. 410:710.
  3. Yuspa SH, et al.. 1989. J Cell Biol. 109:1207.
  4. Roop DR, et al. 1984. J Biol Chem. 259:8037.
  5. Easter SL, et al. 2014. PLoS One. 9:113247. (IF, WB) PubMed
  6. DiTommaso T, et al. 2014. PLoS Genet. 10:1004706. (IHC) PubMed
  7. Li Z, et al. 2007. Cancer Cell. 12:542. (IHC)
  8. Liang Y, 2011 Patholog Res Int. 2011:936794. (IHC) PubMed
  9. Frew IJ, et al. 2008. Mol Cell Biol.28:4536. (IHC) PubMed
  10. Smith BA, et al. 2012. Genes Cancer.3:550. (IHC) PubMed
  11. Johnson JP, et al. 2014 Cancer Geomics Proteomics. 11:115. (IHC) PubMed
  12. Sengupta A, et al. 2010. PLoS One. 5:12249. (IHC) PubMed
  13. Mikaelian I, et al. 2006. Vet Pathol. 43:36. (IHC)
  14. Li W, et al. 2007. J Biol Chem. 282:1161. (IHC, WB) PubMed
  15. Osmanagic-Myers S, et al.2006. J Cell Biol. 174:557. (WB) PubMed
  16. Kittrell FS, et al. 2011. Breast Cancer Res.13:41. (IF) PubMed
  17. Quante T, et al. 2014. Front Oncol. 26:168. (IF) PubMed
  18. Bayo P, Sanchis A, Bravo A, Cascallana JL, Buder K, Tuckermann J, Schütz G, and Pérez P. Glucocorticoid Receptor Is Required for Skin Barrier Competence. Endocrinology, 149: 1377-1388, Mar 2008. (IHC) PubMed
Product Citations
  1. Simigdala N, et al. 2023. Cell Mol Life Sci. 80:100. PubMed
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  3. Murai K, et al. 2022. Nat Commun. 13:6206. PubMed
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  15. Sarper SE, et al. 2019. Dis Model Mech. 12. PubMed
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  18. Xu M, et al. 2019. Am J Transl Res. 11:257. PubMed
  19. Xu KK, et al. 2020. Invest Ophthalmol Vis Sci. 61:30:00. PubMed
  20. Altmeier S, et al. 2016. PLoS Pathog. 12: 1005882. PubMed
  21. Weber MS, et al. 2021. Elife. 10: . PubMed
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  25. Turpin J, et al. 2016. Oncogene. 10.1038/onc.2016.129. PubMed
  26. Osmanagic-Myers S, et al. 2006. J Cell Biol. 174:557-568. PubMed
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  28. Dedeić Z, et al. 2018. Cell Death Differ. 25:1289. PubMed
  29. Liang Y 2011. Patholog Res Int. 2011:936794. PubMed
  30. Kittrell F, et al. 2011. Breast Cancer Res. 13:R41. PubMed
  31. Easter S, et al. 2014. PLoS One. 9:e113247. PubMed
  32. Fang X, et al. 2019. Am J Pathol. 189:1212. PubMed
  33. Reehorst CM, et al. 2021. Development. 148:. PubMed
  34. Buonanno M, et al. 2016. PLoS One. 11: 0138418. PubMed
  35. Li W, et al. 2007. J Biol Chem. 282:1161-1169. PubMed
  36. Jee JP, et al. 2019. Int J Nanomedicine. 14:5449. PubMed
  37. Quante T, et al. 2014. Front Oncol. 4:168. PubMed
  38. Frew I, et al. 2008. Mol Cell Biol. 28:4536-4548. PubMed
  39. Crompton R, et al. 2016. Lab Invest. 10.1038/labinvest.2015.160. PubMed
  40. Shatirishvili M, et al. 2016. Cell Death Dis. 7:e2461. PubMed
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  42. Jozic I, et al. 2021. Commun Biol. 4:757. PubMed
  43. DiTommaso T, et al. 2014. PLoS Genet. 10:e1004706. PubMed
  44. Johnson J, et al. 2014. Cancer Genomics Proteomics. 11:115-126. PubMed
  45. Lin J, et al. 2016. Nature. 540:124-128. PubMed
  46. Shwartz Y, et al. 2020. Cell. 182:578. PubMed
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  48. Norum JH, et al. 2019. Int J Cancer. web only. PubMed
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  50. Anderton H, et al. 2018. Cell Death Differ. 26:877. PubMed
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  52. Alrefai H, et al. 2016. Nat Commun. 7:11724. PubMed
  53. Anderton H, et al. 2017. J Invest Dermatol. 137:2371. PubMed
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RRID
AB_2565052 (BioLegend Cat. No. 905702)
AB_2565052 (BioLegend Cat. No. 905701)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
16687 View all products for this Gene ID
UniProt
View information about Keratin 6A on UniProt.org

Related FAQs

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Go To Top Version: 6    Revision Date: 02/04/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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