- Clone
- SA203G11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ccr2, Ckr2, Ccr2a, Ccr2b
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD192, also known as CCR2, is a 42 kD G-protein coupled receptor that is associated with bone marrow homeostasis. Specifically, CD192 mediates monocyte chemotaxis and acts as a receptor for monocyte chemoattractant protein 1 (MCP-1). CD192 is primarily expressed on monocytes and macrophages, with some expression on basophils. It is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis and cancer.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CCR2 transfectants.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
Antigen Details
- Structure
- G-protein-coupled receptor, 7-transmembrane protein, 42 kD.
- Distribution
-
Monocytes/macrophages and basophils.
- Function
- Mediator of monocyte chemotaxis and bone marrow homeostasis.
- Ligand/Receptor
- MCP-1, Ccl2, Ccl7, and Ccl13.
- Cell Type
- Basophils, Macrophages, Monocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Mack M, et al. 2001. J. Immunol. 166:4697.
2. Dutta P, et al. 2015. Cell Stem Cell. 16:477.
3. Li L, et al. 2008. Kidney Int. 74:1526. - Gene ID
- 12772 View all products for this Gene ID
- UniProt
- View information about CD192 on UniProt.org
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD192 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 647 anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with Ly-6G PE, CD11b FITC, a... -
Brilliant Violet 421™ anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with Ly-6G APC/Cyanine7, CD1... -
PE anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with Ly-6G APC, CD11b PE/Daz... -
PE/Cyanine7 anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with Ly-6G APC, CD11b PE/Daz... -
FITC anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with Ly-6G APC, CD11b PE/Daz... -
Brilliant Violet 605™ anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with Ly-6G (clone 1A8) Alexa... -
Brilliant Violet 650™ anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with Ly-6G (clone 1A8) Alexa... -
Brilliant Violet 785™ anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with Ly-6G (clone 1A8) Alexa... -
Brilliant Violet 510™ anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with Ly-6G (clone 1A8) Alexa... -
Biotin anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with Ly-6G (clone 1A8) Alexa... -
TotalSeq™-A0426 anti-mouse CD192 (CCR2)
-
APC anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with Ly-6G FITC, CD11b PE, a... -
APC/Fire™ 750 anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with Ly-6G FITC, CD11b PE, a... -
TotalSeq™-B0426 anti-mouse CD192 (CCR2)
-
TotalSeq™-C0426 anti-mouse CD192 (CCR2)
-
PE/Cyanine5 anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with anti-mouse Ly-6G FITC, ... -
PE/Dazzle™ 594 anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with anti-mouse Ly-6G FITC, ... -
PE/Fire™ 640 anti-mouse CD192 (CCR2)
C57BL/6 bone marrow cells were stained with anti-mouse Ly-6G... -
Brilliant Violet 711™ anti-mouse CD192 (CCR2)
C57BL/6 mouse bone marrow was stained with anti-mouse Ly-6G ... -
APC/Cyanine7 anti-mouse CD192 (CCR2)
C57BL/6 mouse bone marrow was stained with anti-mouse Ly-6G ... -
PE/Fire™ 700 anti-mouse CD192 (CCR2)
C57BL/6 bone marrow was stained with anti-mouse Ly-6G Brilli... -
PerCP/Fire™ 806 anti-mouse CD192 (CCR2)
C57BL/6 mouse bone marrow Ly6G negative myeloid cells were s... -
PerCP/Fire™ 780 anti-mouse CD192 (CCR2)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Spark Red™ 718 anti-mouse CD192 (CCR2) (Flexi-Fluor™)
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