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APC anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5slash114_APC_011108
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2)APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
  • M5slash114_APC_011108
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2)APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
Cat # Size Price Save
107613 25 µg ¥11,750
107614 100 µg ¥37,210
Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining,the suggested use of this reagent is ≤ 0.25 µg per 106cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  2. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  3. de Souza CO, et al. 2022. J Clin Invest. 132:. PubMed
  4. Zhu C, et al. 2022. Chemotherapy. 67:211. PubMed
  5. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  6. Hao H, et al. 2023. Nat Biomed Eng. . PubMed
  7. Zhuang WR, et al. 2023. Nat Commun. 14:1675. PubMed
  8. Yu H, et al. 2023. Brain Sci. 13:. PubMed
  9. Fu A, et al. 2022. Cell. 185:1356. PubMed
  10. Martin-Martin I, et al. 2022. Cell Rep. 39:110648. PubMed
  11. Ronzier E, et al. 2022. Cells. 11: . PubMed
  12. Zhang Z, et al. 2022. Integr Cancer Ther. 21:15347354221138534. PubMed
  13. An HW, et al. 2022. Cell Rep. 41:111878. PubMed
  14. Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed
  15. Liu G, et al. 2023. Commun Biol. 6:102. PubMed
  16. Sun Y, et al. 2023. Cell Mol Life Sci. 80:63. PubMed
  17. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  18. Kitur K, et al. 2016. Cell Rep. 16:2219-2230. PubMed
  19. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  20. Amir M, et al. 2020. Biochem Biophys Res Commun. 527:1000. PubMed
  21. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  22. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  23. Liu X, et al. 2020. Nature. . PubMed
  24. Rothchild AC, et al. 2020. Bio Protoc. 10:00. PubMed
  25. Shu T, et al. 2022. Eur Respir J. Online ahead of print. PubMed
  26. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  27. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  28. Saito S, et al. 2020. Nutrients. 12:. PubMed
  29. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
  30. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  31. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  32. Lee JH, et al. 2022. iScience. 25:104166. PubMed
  33. Yeung AK, et al. 2020. Blood Adv. 4:6204. PubMed
  34. Teng ZX, et al. 2022. J Inflamm Res. 15:3187. PubMed
  35. Park S, et al. 2021. Nat Cell Biol. 23:476. PubMed
  36. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  37. Rayamajhi M, et al. 2010. J Exp Med. 207:327. PubMed
  38. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  39. Müller A, et al. 2020. J Clin Invest. 5765:130. PubMed
  40. Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed
  41. Gray D, et al. 2007. J Exp Med. 204:2521. PubMed
  42. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  43. Al Mamun A, et al. 2019. Neurochem Int. 127:148. PubMed
  44. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  45. Si Y, et al. 2018. J Control Release. 282:120. PubMed
  46. Xu X, et al. 2018. Cell. 175:1336. PubMed
  47. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  48. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  49. Van Winkle JA et al. 2018. Cell host & microbe. 24(5):665-676 . PubMed
  50. Levesque S, et al. 2019. Oncoimmunology. 8:e1657375. PubMed
  51. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  52. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  53. Jorapur A, et al. 2022. PLoS Pathog. 18:e1010200. PubMed
  54. Juncheng P, et al. 2022. J Immunother Cancer. 10:. PubMed
  55. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  56. Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed
  57. Unudurthi SD, et al. 2020. Life Sci. 117440:247. PubMed
  58. Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed
  59. Boucard-Jourdin M, et al. 2016. J Immunol. 197: 1968 - 1978. PubMed
  60. Milner MT, et al. 2022. STAR Protoc. 3:101295. PubMed
  61. Han F, et al. 2020. Invest Ophthalmol Vis Sci. 61:24. PubMed
  62. Mattila JT, et al. 2017. J Immunol. 199:806. PubMed
  63. Zhu W, et al. 2019. FASEB J. 33:5208. PubMed
  64. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
  65. Morikawa M, et al. 2016. PLoS One. 11:e0163607. PubMed
  66. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  67. Kitur K, et al. 2015. PLoS Pathog. 11:1004820. PubMed
  68. Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed
  69. Liu P, et al. 2020. Mol Cell. 748:77. PubMed
  70. Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed
  71. Meunier I, et al. 2015. PLoS One. 10: 0138055. PubMed
  72. Zhao H, et al. 2022. Commun Biol. 5:74. PubMed
  73. Dheilly E, et al. 2020. Cancer Cell. 37(5):674-689. PubMed
  74. Tassi I, et al. 2014. J Immunol. 193:4303. PubMed
  75. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  76. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  77. Fitzpatrick Z, et al. 2020. Nature. 587:472. PubMed
  78. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  79. Zhu Y, et al. 2021. Int J Oral Sci. 13:25. PubMed
  80. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  81. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  82. Goc J, et al. 2021. Cell. 184:5015. PubMed
  83. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  84. Mangal JL, et al. 2020. J Mater Chem B. 8:5195. PubMed
  85. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  86. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  87. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  88. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
RRID
AB_313328 (BioLegend Cat. No. 107613)
AB_313328 (BioLegend Cat. No. 107614)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
I-A/I-E
Specificity Alt (DOES NOT SHOW ON TDS):
I-A/I-E
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about I-A/I-E on UniProt.org

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 4    Revision Date: 10/30/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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