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APC/Cyanine7 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_APCCyanine7_CD19_Antibody_031819_updated.png
C57BL/6 splenocytes were stained with CD3 FITC and CD19 (clone 6D5) APC/Cyaninie7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
  • 6D5_APCCyanine7_CD19_Antibody_031819_updated.png
    C57BL/6 splenocytes were stained with CD3 FITC and CD19 (clone 6D5) APC/Cyaninie7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Save
115529 25 µg ¥24,640
115530 100 µg ¥50,380
Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  2. Pani F, et al. 2021. Endocrinology. 162:. PubMed
  3. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  4. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  5. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  6. Laffleur B, et al. 2022. Sci Immunol. 7:eabn2738. PubMed
  7. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  8. Backlund C, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204078119. PubMed
  9. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  10. Laidlaw BJ, et al. 2020. Nat Immunol. 1.626388889. PubMed
  11. Pérez-Torrado V, et al. 2022. Bio Protoc. 12:. PubMed
  12. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  13. Zhang Z, et al. 2023. Aging Dis. 14:966. PubMed
  14. Lütge M, et al. 2023. Nat Immunol. . PubMed
  15. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  16. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  17. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  18. Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
  19. Machata S, et al. 2021. Front Immunol. 11:565869. PubMed
  20. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  21. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  22. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
  23. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  24. Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
  25. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  26. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  27. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  28. Tang J, et al. 2018. Mol Cell Biol. 38:e00103. PubMed
  29. Quatrini L, et al. 2018. Nat Immunol. 19:954. PubMed
  30. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  31. Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed
  32. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  33. Trott DW, et al. 2021. Geroscience. 43:1331. PubMed
  34. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  35. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  36. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  37. Bresque M, et al. 2022. J Biol Chem. :101711. PubMed
  38. Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed
  39. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  40. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  41. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  42. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  43. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  44. Stone AEL, et al. 2019. Nat Commun. 10:3649. PubMed
  45. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  46. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
  47. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  48. Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed
  49. Wei SC, et al. 2020. Cancer Discov. . PubMed
  50. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  51. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  52. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  53. Kim P, et al. 2015. J Exp Med. 212:633. PubMed
  54. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  55. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  56. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  57. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  58. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
  59. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  60. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  61. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  62. Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed
  63. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
  64. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  65. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  66. Xu J et al. 2018. Cell. 173(3):762-775 . PubMed
  67. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  68. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  69. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  70. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  71. Carbone C, et al. 2021. J Immunother Cancer. 9:. PubMed
  72. Elkhatib SK, et al. 2021. Biol Psychiatry Glob Open Sci. 1:190. PubMed
  73. Colombo M, et al. 2022. iScience. 25:105042. PubMed
  74. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  75. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  76. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  77. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  78. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  79. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  80. Yahagi A, et al. 2019. RMD Open. 5:e000853. PubMed
  81. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  82. Fischer FA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  83. Trott DW, et al. 2021. J Physiol. 599:3973. PubMed
  84. Engblom C, et al. 2017. Science. 358:6367. PubMed
  85. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  86. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  87. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  88. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  89. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
  90. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  91. Du P, et al. 2022. Front Pharmacol. 13:805508. PubMed
  92. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
  93. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  94. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  95. La Salvia S, et al. 2020. Am J Physiol Renal Physiol. 319:F868. PubMed
  96. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  97. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  98. Strawbridge R, et al. 2016. Diabetes. 65: 2888 - 2899. PubMed
  99. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  100. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  101. Chavez JS, et al. 2022. Cells. 11:. PubMed
  102. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  103. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  104. Wilson CS, et al. 2021. JCI Insight. 6:. PubMed
  105. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  106. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
  107. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  108. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  109. Cheng Q, et al. 2020. Eur J Immunol. 50:284. PubMed
  110. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
RRID
AB_830706 (BioLegend Cat. No. 115529)
AB_830706 (BioLegend Cat. No. 115530)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD19
Specificity Alt (DOES NOT SHOW ON TDS):
CD19
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 3    Revision Date: 03/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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