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APC/Fire™ 810 anti-human CD25 Antibody

Pricing & Availability
Clone
M-A251 (See other available formats)
Regulatory Status
RUO
Workshop
IV A053
Other Names
IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M-A251_APCFire810_CD25_Antibody_1_092520
PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with anti-human CD4 Brilliant Violet 421™ and anti-human CD25 APC/Fire™ 810 (clone M-A251) (left) or stained with CD4 Brilliant Violet 421™ only (right).
  • M-A251_APCFire810_CD25_Antibody_1_092520
    PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with anti-human CD4 Brilliant Violet 421™ and anti-human CD25 APC/Fire™ 810 (clone M-A251) (left) or stained with CD4 Brilliant Violet 421™ only (right).
  • M-A251_APCFire810_CD25_Antibody_2_092520
    Human peripheral blood lymphocytes were stained with CD4 Brilliant Violet 421™ and anti-human CD25 APC/Fire™ 810 (clone M-A251) (left) or stained with CD4 Brilliant Violet 421™ only (right).
Compare all formats See APC/Fire™ 810 spectral data
Cat # Size Price Save
356149 25 tests ¥34,980
356150 100 tests ¥77,440
Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PHA-induced lymphocyte cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections1 and spatial biology (IBEX)2,3.

The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.

Application References

(PubMed link indicates BioLegend citation)
  1. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  2. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  3. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID
AB_2876679 (BioLegend Cat. No. 356149)
AB_2876679 (BioLegend Cat. No. 356150)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD; low-affinity IL-2 receptor α chain
Distribution

Activated T and B cells, monocytes/macrophages, Tregs

Interaction
Associates with IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form a high-affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD25
Specificity Alt (DOES NOT SHOW ON TDS):
CD25
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD25 on UniProt.org

Other Formats

View All CD25 Reagents Request Custom Conjugation
Description Clone Applications
APC/Cyanine7 anti-human CD25 M-A251 FC
Purified anti-human CD25 M-A251 FC,IHC-F
PE anti-human CD25 M-A251 FC
FITC anti-human CD25 M-A251 FC
PE/Cyanine7 anti-human CD25 M-A251 FC
APC anti-human CD25 M-A251 FC
PerCP/Cyanine5.5 anti-human CD25 M-A251 FC
Brilliant Violet 421™ anti-human CD25 M-A251 FC
Alexa Fluor® 488 anti-human CD25 M-A251 FC
Alexa Fluor® 700 anti-human CD25 M-A251 FC
Brilliant Violet 510™ anti-human CD25 M-A251 FC
PE/Dazzle™ 594 anti-human CD25 M-A251 FC
Biotin anti-human CD25 M-A251 FC
Alexa Fluor® 647 anti-human CD25 M-A251 FC,SB
Pacific Blue™ anti-human CD25 M-A251 FC
PerCP anti-human CD25 M-A251 FC
APC/Fire™ 750 anti-human CD25 M-A251 FC
Brilliant Violet 711™ anti-human CD25 M-A251 FC
Brilliant Violet 785™ anti-human CD25 M-A251 FC
Brilliant Violet 605™ anti-human CD25 M-A251 FC
KIRAVIA Blue 520™ anti-human CD25 M-A251 FC
PE/Fire™ 700 anti-human CD25 M-A251 FC
APC/Fire™ 810 anti-human CD25 M-A251 FC
Spark NIR™ 685 anti-human CD25 Antibody M-A251 FC
Spark YG™ 581 anti-human CD25 M-A251 FC
PE/Fire™ 640 anti-human CD25 Antibody M-A251 FC
PE anti-human CD25 M-A251 FC
PerCP/Cyanine5.5 anti-human CD25 M-A251 FC
APC/Fire™ 750 anti-human CD25 M-A251 FC
PE/Cyanine7 anti-human CD25 M-A251 FC
APC anti-human CD25 M-A251 FC
PE/Cyanine5 anti-human CD25 M-A251 FC
FITC anti-human CD25 M-A251 FC
Spark Red™ 718 anti-human CD25 M-A251 FC
GMP PE anti-human CD25 M-A251 FC
GMP PE/Cyanine7 anti-human CD25 M-A251 FC
PerCP/Fire™ 780 anti-human CD25 M-A251 FC
PE/Fire™ 744 anti-human CD25 M-A251 FC
PerCP/Fire™ 806 anti-human CD25 M-A251 FC
Brilliant Violet 650™ anti-human CD25 M-A251 FC
Brilliant Violet 750™ anti-human CD25 M-A251 FC
Go To Top Version: 1    Revision Date: 09/11/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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