APC/Fire™ 810 anti-mouse CD3 Antibody, CD3, 17A2

Clone: 17A2 (See other available formats)
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Rat IgG2b, κ
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C57BL/6 splenocytes were stained with CD45R/B220 PE and CD3 (clone 17A2) APC/Fire™ 810 (left) or rat IgG2b, κ APC/Fire™ 810 isotype control (right).

Compare all formats See APC/Fire™ 810 spectral data

Cat #	Size	Price	Save
100267	25 µg	¥28,380
100268	100 µg	¥77,440

Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: γδTCR-positive T-T hybridoma D1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)^1,2.
RRID: AB_2876392 (BioLegend Cat. No. 100267)
AB_2876392 (BioLegend Cat. No. 100268)

Antigen Details

Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC-complex
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12502 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):: CD3
Specificity Alt (DOES NOT SHOW ON TDS):: CD3
App Abbreviation (DOES NOT SHOW ON TDS):: FC
UniProt: View information about CD3 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
FITC anti-mouse CD3	17A2	FC
PE anti-mouse CD3	17A2	FC
Purified anti-mouse CD3	17A2	FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3	17A2	FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3	17A2	FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3	17A2	FC
Alexa Fluor® 700 anti-mouse CD3	17A2	FC
PerCP/Cyanine5.5 anti-mouse CD3	17A2	FC
PE/Cyanine7 anti-mouse CD3	17A2	FC
APC/Cyanine7 anti-mouse CD3	17A2	FC
Brilliant Violet 421™ anti-mouse CD3	17A2	FC,ICC
Brilliant Violet 570™ anti-mouse CD3	17A2	FC
Brilliant Violet 650™ anti-mouse CD3	17A2	FC
Brilliant Violet 785™ anti-mouse CD3	17A2	FC
Brilliant Violet 510™ anti-mouse CD3	17A2	FC
APC anti-mouse CD3	17A2	FC
Ultra-LEAF™ Purified anti-mouse CD3	17A2	FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3	17A2	FC
Alexa Fluor® 594 anti-mouse CD3	17A2	IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3	17A2	FC
Biotin anti-mouse CD3	17A2	FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3	17A2	FC
APC/Fire™ 750 anti-mouse CD3	17A2	FC
Brilliant Violet 750™ anti-mouse CD3	17A2	FC
TotalSeq™-A0182 anti-mouse CD3	17A2	PG
TotalSeq™-B0182 anti-mouse CD3	17A2	PG
Spark Blue™ 550 anti-mouse CD3	17A2	FC
Spark NIR™ 685 anti-mouse CD3	17A2	FC
TotalSeq™-C0182 anti-mouse CD3	17A2	PG
APC/Fire™ 810 anti-mouse CD3	17A2	FC
PE/Fire™ 640 anti-mouse CD3	17A2	FC
Spark YG™ 570 anti-mouse CD3	17A2	IHC-F
PE/Fire™ 700 anti-mouse CD3	17A2	FC
PE/Cyanine5 anti-mouse CD3	17A2	FC
Spark Blue™ 574 anti-mouse CD3 Antibody	17A2	FC
Spark Violet™ 423 anti-mouse CD3	17A2	FC
PE/Fire™ 810 anti-mouse CD3	17A2	FC
Spark Red™ 718 anti-mouse CD3	17A2	FC
Spark UV™ 387 anti-mouse CD3	17A2	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 06/05/2020

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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