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Brilliant Violet 421™ anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
1-6D5_BV421_032911
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • 1-6D5_BV421_032911
    C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • 2-6D5_BV421_CD19_Antibody_2_041619
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 2.5 µg/ml of CD19 (clone 6D5) Brilliant Violet 421TM, 2.5 µg/ml of CD3 (clone 17A2) Alexa Fluor® 594 (red) and 2.5 µg/ml of CD11b (clone M1/70) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Save
115537 125 µL ¥36,300
115549 50 µg ¥55,660
115538 500 µL ¥82,280
Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemistry on Frozen tissue using the µg size, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed
  2. Ostendorf BN, et al. 2022. Nature. 611:346. PubMed
  3. Nettersheim FS, et al. 2023. Front Cardiovasc Med. 9:1076808. PubMed
  4. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  5. Bermea KC, et al. 2022. J Vis Exp. . PubMed
  6. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  7. Caradonna TM, et al. 2022. Cell Rep. 41:111628. PubMed
  8. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  9. Doloff JC, et al. 2023. Sci Adv. 9:eade9488. PubMed
  10. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  11. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  12. Jiang W, et al. 2017. Sci Rep. 7:6501. PubMed
  13. Rosalia R, et al. 2013. Immunobiology. 218:851. PubMed
  14. Yilmaz B, et al. 2021. Cell Host Microbe. 29(4):650-663.e9. PubMed
  15. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  16. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  17. Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed
  18. Park JG, et al. 2021. iScience. 24(9):102941. PubMed
  19. Zhang X, et al. 2022. Pharmaceutics. 14:. PubMed
  20. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  21. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  22. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  23. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  24. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
  25. Webster P, et al. 2018. Nat Commun. 9:2649. PubMed
  26. Lee CG, et al. 2019. JCI Insight. 4:5. PubMed
  27. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  28. Zheng Z, et al. 2020. Cell Reports. 31(13):107819. PubMed
  29. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  30. Prooyen N, et al. 2016. PLoS One. 12: 1005749. PubMed
  31. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  32. Dibra D, et al. 2016. Clin Cancer Res. 22: 3876 - 3883. PubMed
  33. Bello A, et al. 2021. Immunol Cell Biol. 99:879. PubMed
  34. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  35. Giampazolias E, et al. 2021. Cell. . PubMed
  36. He C, et al. 2022. Nat Commun. 13:5459. PubMed
  37. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
  38. Kerkhofs D, et al. 2020. Theranostics. 7.022222222. PubMed
  39. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  40. Akoolo L, et al. 2021. Cell Microbiol. 23:e13350. PubMed
  41. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  42. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  43. Kelley WJ, et al. 2022. JCI Insight. 7: . PubMed
  44. Jolly A, et al. 2022. Cell Rep Methods. 2:100315. PubMed
  45. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  46. Monzon-Casanova E, et al. 2021. Eur J Immunol. 51:2266. PubMed
  47. Wang Y, et al. 2020. Immunity. 53(6):1151-1167.e6. PubMed
  48. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  49. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  50. Steinmetz TD, et al. 2021. Autophagy. 17:2238. PubMed
  51. Schaum N, et al. 2020. Nature. 583:596. PubMed
  52. Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
RRID
AB_10895761 (BioLegend Cat. No. 115537)
AB_10895761 (BioLegend Cat. No. 115549)
AB_10895761 (BioLegend Cat. No. 115538)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD19
Specificity Alt (DOES NOT SHOW ON TDS):
CD19
App Abbreviation (DOES NOT SHOW ON TDS):
FC,IHC-F
UniProt
View information about CD19 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 3    Revision Date: 04/16/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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