- Clone
- 1B4/CD18 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Integrin beta-2, integrin beta chain, beta 2
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were stained with purified CD18 (clone 1B4/CD18) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram) followed by PE Goat anti-mouse IgG.
CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human peripheral blood lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The 1B4/CD18 antibody was able to partially block the Ts1/18 antibody, indicating the two anti-CD18 antibodies recognize distinct but overlapping epitopes.
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Application References
(PubMed link indicates BioLegend citation) -
- Singer II, et al. 1993. J. Immunol. 150:2844.
- Seth R, et al. 1991. FEBS Lett. 282:193.
- RRID
-
AB_2650942 (BioLegend Cat. No. 373402)
Antigen Details
- Structure
- Integrin, type I transmembrane glycoprotein
- Distribution
-
Leukocytes
- Function
- Adhesion and signaling in hematopoietic cells
- Interaction
- Associates with CD11a, CD11b, or CD11c
- Ligand/Receptor
- ICAMs
- Cell Type
- Leukocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Hogg N, et al. 1999. J. Clin. Invest. 103:97.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Springer TA. 1994. Cell 76:301. - Gene ID
- 3689 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD18
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD18
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about CD18 on UniProt.org
Related FAQs
Other Formats
View All CD18 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD18 | 1B4/CD18 | FC |
| APC anti-human CD18 | 1B4/CD18 | FC |
| PE/Cyanine7 anti-human CD18 | 1B4/CD18 | FC |
| Alexa Fluor® 488 anti-human CD18 | 1B4/CD18 | FC |
| PE anti-human CD18 | 1B4/CD18 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD18
Human peripheral blood lymphocytes were stained with purifie... -
APC anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
PE/Cyanine7 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
Alexa Fluor® 488 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
PE anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c...
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