Purified anti-mouse CD3 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

γδTCR-positive T-T hybridoma D1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IHC-F - Verified
IP, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)^1,2.

Application References

(PubMed link indicates BioLegend citation)

1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
2. Abdel-Gadir A, et al. 2019. Nat Med. 25:1164. PubMed
3. Hamada T, et al. 2008. J Immunol. 180:1843. PubMed
4. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
5. Bernstein DL, et al. 2020. Brain Behav Immun. 87:543. PubMed
6. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
7. Doll JR, et al. 2020. PLoS Pathog. 16:e1008296. PubMed
8. Li TT, et al. 2021. BMC Immunol. 22:55. PubMed
9. Lu X, et al. 2021. J Exp Med. 218: . PubMed
10. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
11. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
12. Campisi L, et al. 2022. Nature. 606:945. PubMed
13. Halin C, et al. 2022. EMBO J. 41:e111528. PubMed
14. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
15. Zhao J, et al. 2022. J Clin Invest. 132: . PubMed
16. Glaubitz J, et al. 2023. Gut. :. PubMed
17. Fueyo-González F, et al. 2022. Immunity. 55:459. PubMed
18. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
19. Robles-Valero J, et al. 2022. Mol Oncol. 16:3533. PubMed
20. Mammadli M, et al. 2023. Int J Mol Sci. 24:. PubMed
21. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
22. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
23. Kulkarni NN, et al. 2020. J Invest Dermatol. 140:645. PubMed
24. Boyd DF, et al. 2020. Nature. 587:466. PubMed
25. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
26. Bergstrom K, et al. 2008. Infect Immun. 76:796. PubMed
27. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
28. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
29. Hawse WF, et al. 2019. J Immunol. 203:2771. PubMed
30. Zhao J, et al. 2021. JCI Insight. 6:. PubMed
31. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
32. Xu L, et al. 2017. Int J Biol Macromol. . 10.1016/j.ijbiomac.2017.07.090. PubMed
33. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
34. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
35. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
36. Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
37. Rasoulouniriana D, et al. 2019. J Clin Invest. 129:4151. PubMed
38. Thulasingam S, et al. 2019. Int J Mol Sci. 20. PubMed
39. Haque M, et al. 2021. Viruses. 13:. PubMed
40. Wabitsch S, et al. 2021. STAR Protoc. 2:100517. PubMed
41. Schafflick D, et al. 2020. Nat Commun. 247:11. PubMed
42. Kerdidani D, et al. 2019. Nat Commun. 10:1405. PubMed
43. Hayashi Y, et al. 2017. J Cancer. 8:2478. PubMed
44. Düsedau HP, et al. 2019. Glia. 67:193. PubMed
45. Liu T, et al. 2020. Sci Adv. 6:eaay1497. PubMed
46. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
47. Boothby IC, et al. 2021. Nature. 599:667. PubMed
48. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
49. Chen Y, et al. 2018. Oncoimmunology. 7:e1388484. PubMed
50. Child KM et al. 2018. The Journal of Neuroscience. 38(31):6806-6824 . PubMed
51. Recino A, et al. 2019. Gene Ther. 26:40. PubMed
52. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
53. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
54. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
55. Adamson SE, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1020. PubMed
56. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
57. Yoshihara T, et al. 2020. Gut Microbes. 11:1385. PubMed
58. Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed
59. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
60. Karhadkar TR, et al. 2021. PLoS One. 16:e0245924. PubMed
61. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
62. He LD, et al. 2021. EMBO Rep. 22:e52196. PubMed
63. Forsyth KS, et al. 2020. PLoS Pathog. 16:e1008685. PubMed
64. Srey MT, et al. 2020. PLoS Negl Trop Dis. 14:e0008842. PubMed
65. Kopec A, et al. 2014. Toxicol Sci. 141:515. PubMed
66. Mainini F, et al. 2018. Nucleic Acid Ther. 28:225. PubMed
67. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
68. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
69. Wei S, et al. 2019. Virulence. 0.588888889. PubMed
70. Miret JJ, et al. 2019. J Immunother Cancer. 7:32. PubMed
71. Bittner-Eddy PD, et al. 2020. Front Immunol. 11:677. PubMed
72. Dooley K, et al. 2021. Mol Ther. 29:1729. PubMed
73. Liu M, et al. 2018. Diabetologia. 61:2333. PubMed
74. Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed
75. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
76. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
77. Lim CS, et al. 2020. Front Immunol. 11:1186. PubMed
78. You X, et al. 2022. Front Genet. 12:790990. PubMed
79. Karhadkar TR, et al. 2020. bioRxiv. . PubMed
80. Clark K, et al. 2019. Front Immunol. 10:2355. PubMed
81. Gilfillan CB, et al. 2020. Eur J Immunol. 50:505. PubMed
82. Fitzpatrick E, et al. 2017. PLoS One. 10.1371/journal.pone.0169273. PubMed
83. Karhadkar TR, et al. 2020. Am J Physiol Lung Cell Mol Physiol. 318:L165. PubMed
84. Sugita S, et al. 2017. Arthritis Res Ther. 19:176. PubMed
85. Patterson AR, et al. 2019. J Allergy Clin Immunol. 143:245. PubMed
86. Williams SK, et al. 2018. Sci Rep. 8:13628. PubMed
87. Bartolacci C, et al. 2018. Cancer Immunol Res. 1.281944444. PubMed
88. Zelco A, et al. 2020. Front Cell Neurosci. 0.75625. PubMed
89. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
90. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
91. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
92. Moon H, et al. 2019. Nat Commun. 10:2225. PubMed
93. Levy BD, et al. 2020. J Allergy Clin Immunol. 145:335. PubMed
94. Flietner E, et al. 2022. Sci Rep. 12:10616. PubMed
95. Pan S, et al. 2021. Front Cell Dev Biol. 9:629306. PubMed
96. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
97. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
98. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
99. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
100. Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
101. Gaspar M, et al. 2020. Cancer Immunol Res. 0.875694444. PubMed
102. Tilley S, et al. 2007. J Immunol . 178:3208. PubMed
103. Kang LJ, et al. 2020. Sci Rep. 10:5603. PubMed
104. Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed
105. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
106. Di Dalmazi G, et al. 2019. J Immunol. 202:1350. PubMed
107. Lee YJ, et al. 2018. Front Microbiol. 9:83. PubMed
108. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
109. Heib T, et al. 2021. Cell Reports. 35(6):109102. PubMed
110. He Y, et al. 2021. eLife. 10:00. PubMed
111. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
112. Katsuyama T, et al. 2019. J Clin Invest. 129:5411. PubMed
113. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
114. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
115. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
116. Gonzales C, et al. 2016. PLoS One. 11:e0164557. PubMed
117. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
118. Rastelli D, et al. 2021. J Clin Invest. :e150789. PubMed
119. Moretti FA, et al. 2021. Sci Rep. 11:23221. PubMed
120. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
121. Robles-Valero J, et al. 2021. EMBO J. 40:e108125. PubMed
122. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
123. Yadav A, et al. 2009. Am J Physiol Renal Physiol. 298:734. PubMed
124. Shao Y, et al. 2017. Onco Targets Ther. 10:2675. PubMed
125. Egaña–Gorroño L, et al. 2019. Atherosclerosis. 289:184. PubMed
126. Regan–Komito D, et al. 2017. Front Immunol. 1.459027778. PubMed
127. He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed
128. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed

See More

RRID

AB_312658 (BioLegend Cat. No. 100201)
AB_312658 (BioLegend Cat. No. 100202)

Antigen Details

Structure

Ig superfamily, CD3/TCR, 20 kD

Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function

Antigen recognition, TCR signal transduction, T cell activation

Ligand/Receptor

Peptide antigen/MHC-complex

Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID

12502 View all products for this Gene ID

Specificity (DOES NOT SHOW ON TDS):

CD3

Specificity Alt (DOES NOT SHOW ON TDS):

CD3

App Abbreviation (DOES NOT SHOW ON TDS):

FC,IHC-F,IP,ICC

UniProt

View information about CD3 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis