Purified anti-mouse CD3 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

γδTCR-positive T-T hybridoma D1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IHC-F - Verified
IP, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)^1,2.

Application References

(PubMed link indicates BioLegend citation)

1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

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See More

RRID

AB_312658 (BioLegend Cat. No. 100201)
AB_312658 (BioLegend Cat. No. 100202)

Antigen Details

Structure

Ig superfamily, CD3/TCR, 20 kD

Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function

Antigen recognition, TCR signal transduction, T cell activation

Ligand/Receptor

Peptide antigen/MHC-complex

Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID

12502 View all products for this Gene ID

Specificity (DOES NOT SHOW ON TDS):

CD3

Specificity Alt (DOES NOT SHOW ON TDS):

CD3

App Abbreviation (DOES NOT SHOW ON TDS):

FC,IHC-F,IP,ICC

UniProt

View information about CD3 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis