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Alexa Fluor® 647 anti-Bcl-2 Antibody

Pricing & Availability
Clone
BCL/10C4 (See other available formats)
Regulatory Status
RUO
Other Names
B-cell leukemia 2
Isotype
Mouse IgG1, κ
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Product Citations
publications
BCL10C4_A647_030510
C57BL/6 splenocytes intracellularly stained with BCL/10C4 Alexa Fluor® 647. Cells were fixed and permeabilized with FOXP3 Fix/Perm Buffer Set.
  • BCL10C4_A647_030510
    C57BL/6 splenocytes intracellularly stained with BCL/10C4 Alexa Fluor® 647. Cells were fixed and permeabilized with FOXP3 Fix/Perm Buffer Set.
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Save
633509 25 µg ¥28,380
633510 100 µg ¥66,000
Description

Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, the nuclear envelope, and the endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
N-terminal, Amino acid residues 61-76 of mouse Bcl-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue4, immunocytochemical staining5, and immunoprecipitation5.

Application References

(PubMed link indicates BioLegend citation)
  1. Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
  2. Zuber J, et al. 2011. Genes Dev. 25:1628. (WB) PubMed
  3. Doi K, et al. 2012. Biochem Biophys Res Commun. 425:107. PubMed
  4. Yamanaka, et al. 2003. J. Immunol. 170:816. (IHC)
  5. Conus S, et al. 2000. EMBO J. 19:1534. (WB, IP, ICC)
Product Citations
  1. Grebinoski S, et al. 2022. Nat Immunol. 23:868. PubMed
  2. Shallberg LA, et al. 2022. PLoS Pathog. 18:e1010296. PubMed
  3. VanDyke D, et al. 2022. Cell Rep. 41:111478. PubMed
  4. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  5. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  6. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  7. Bauché D et al. 2018. Immunity. 49(2):342-352 . PubMed
  8. Su W, et al. 2020. Cell Metabolism. 32(6):996-1011.e7. PubMed
  9. Wiede F, et al. 2021. Cancer Discov. Online ahead of print. PubMed
  10. Yang HL, et al. 2020. Am J Reprod Immunol. 84:e13261. PubMed
  11. Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed
  12. Beus J, et al. 2013. PLoS One. 8:71221. PubMed
  13. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  14. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  15. Zhu X et al. 2019. Cell reports. 27(4):1205-1220 . PubMed
  16. Severance AL, et al. 2022. iScience. 25:104400. PubMed
  17. Huang H, et al. 2021. Cell. 184(5):1245-1261.e21. PubMed
  18. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
  19. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  20. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  21. Wang H, et al. 2016. PLoS Pathog. 12: 1005787. PubMed
  22. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
RRID
AB_2064149 (BioLegend Cat. No. 633509)
AB_2064149 (BioLegend Cat. No. 633510)

Antigen Details

Structure
Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
Distribution

Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum

Function
Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
Modification
Phosphorylation, Ubiquitination
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References

1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214.
2. Yang E, et al. 1995. Cell 80:285.
3. Huang Z, et al. 2000. Oncogene 19:6627.
4. Deng X, et al. 2003. Blood. 102:3179.

Regulation
Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
Gene ID
596 View all products for this Gene ID
UniProt
View information about Bcl-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09/11/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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