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Alexa Fluor® 647 anti-human IL-6 Antibody

Pricing & Availability
Clone
MQ2-13A5 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Isotype
Rat IgG1, κ
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Product Citations
publications
MQ2-13A5_A647_IL-6_Antibody_041219
LPS-stimulated human peripheral blood mononuclear cells (6 hours) were fixed and permeabilized then intracellularly stained with CD14 FITC and anti-human IL-6 (clone MQ2-13A5) Alexa Fluor® 647 (left), or rat IgG1, κ Alexa Fluor® 647 isotype control (right).
  • MQ2-13A5_A647_IL-6_Antibody_041219
    LPS-stimulated human peripheral blood mononuclear cells (6 hours) were fixed and permeabilized then intracellularly stained with CD14 FITC and anti-human IL-6 (clone MQ2-13A5) Alexa Fluor® 647 (left), or rat IgG1, κ Alexa Fluor® 647 isotype control (right).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Save
501123 25 tests ¥34,980
501124 100 tests ¥76,340
Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-7- expressed, recombinant human IL-6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Capture1-3,7: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202) as the detecting antibody.

Neutralization1-3,5,6: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110). The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.

Additional applications (for the relevant formats) include: intracellular flow cytometry10.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
  3. Gaines Das R, et al. 1993. J. Immunol. Methods 160:147.
  4. Enriquez J, et al. 2002. Adv. Perit Dial. 18:177.
  5. Zou JP, et al. 1999. J. Immunol. 162:4882.
  6. Wyant TL, et al. 1999. Infect. Immun. 67:1338.
  7. Lesmeister MJ, et al. 2005. Reprod. Biol. Endocrinol. 3:74.
  8. Terasaka Y, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:2441 PubMed
  9. Girndt M, et al. 1998. J. Am. Soc. Nephrol. 9:1689.
Product Citations
  1. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
RRID
AB_2810624 (BioLegend Cat. No. 501123)
AB_2810624 (BioLegend Cat. No. 501124)

Antigen Details

Structure
Neuropoietic cytokine family; 26 kD (Mammalian)
Bioactivity
Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets
T cells, B cells, hepatocytes, cholinergic neurons
Receptors
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Int. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. van Oers M, et al. 1993. Ann. Hematol. 66:219.

Regulation
Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, and IL-17; downregulated by glucocorticoids IL-4 and TGF-β
Gene ID
3569 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Other Formats

View All IL-6 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-human IL-6 MQ2-13A5 ICFC
PE anti-human IL-6 MQ2-13A5 ICFC
Purified anti-human IL-6 MQ2-13A5 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC
APC anti-human IL-6 MQ2-13A5 ICFC
Pacific Blue™ anti-human IL-6 MQ2-13A5 ICFC
Purified anti-human IL-6 (Maxpar® Ready) MQ2-13A5 ELISA Capture,CyTOF®
PerCP/Cyanine5.5 anti-human IL-6 MQ2-13A5 ICFC
PE/Cyanine7 anti-human IL-6 MQ2-13A5 ICFC
Alexa Fluor® 647 anti-human IL-6 MQ2-13A5 ICFC
PE/Dazzle™ 594 anti-human IL-6 MQ2-13A5 ICFC
Ultra-LEAF™ Purified anti-human IL-6 MQ2-13A5 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC
TotalSeq™-B0942 anti-human IL-6 MQ2-13A5 ICPG
TotalSeq™-C0942 anti-human IL-6 MQ2-13A5 ICPG
Go To Top Version: 1    Revision Date: 04/12/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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