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APC anti-rat CD47 Antibody

Pricing & Availability
Clone
OX-101 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin-associated protein, IAP, neurophilin,gp42, Rh-associated protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-101_APC_CD47_Antibody_111418
LOU rat splenocytes were surface stained with CD47 (clone OX-101) APC (filled histogram), or Mouse IgG1, κ APC Isotype Control (open histogram).
  • OX-101_APC_CD47_Antibody_111418
    LOU rat splenocytes were surface stained with CD47 (clone OX-101) APC (filled histogram), or Mouse IgG1, κ APC Isotype Control (open histogram).
Compare all formats See APC spectral data
Cat # Size Price Save
204113 25 µg ¥28,820
204114 100 µg ¥53,460
Description

CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment, and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. The significance of this molecule is recently drawing increasing attention. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. DeVries HE, et al. 2002. J. Immunol. 168:5832.
RRID
AB_2783142 (BioLegend Cat. No. 204113)
AB_2783142 (BioLegend Cat. No. 204114)

Antigen Details

Structure
50 kD plasma membrane protein of immunoglobulin supergene family, with an extracellular IgV-like domain of 120 amino acids, a five membrane spanning segment of 152 amino acids, and a short cytoplasmic tail of 30 amino acids.
Distribution

Broad tissue distribution, including haemopoietic cells, epithelial and endothelial cells, fibroblasts, erythrocytes and other tissue cells.

Function
Adhesion molecules, immune regulation
Interaction
Associate with β3 integrins
Ligand/Receptor
SIRPα (CD172α, signal regulatory protein alpha), thrombospondin
Cell Type
Endothelial cells, Epithelial cells, Erythrocytes, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Vernon-Wilson, et al. 2000. Eur. J. Immunol. 30:2130.
  2. Brown E, et al. 1990. J. Cell. Biol. 111:2785.
  3. Mawby WJ, et al. 1994. Biochem. J. 304:525.
Gene ID
16423 View all products for this Gene ID
UniProt
View information about CD47 on UniProt.org
Go To Top Version: 1    Revision Date: 11/15/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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