Loading...

APC/Cyanine7 anti-mouse CD3ε Antibody

Pricing & Availability
Clone
145-2C11 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
145-2c11_APC_091009
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) APC/Cyanine7 (filled histogram) or Armenian hamster IgG APC/Cyanine7 isotype control (open histogram).
  • 145-2c11_APC_091009
    C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) APC/Cyanine7 (filled histogram) or Armenian hamster IgG APC/Cyanine7 isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Save
100329 25 µg ¥17,450
100330 100 µg ¥38,270
Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32,  and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
  3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
  4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
  7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
  8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
  10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
  11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
  13. Ko SY, et al. 2005. J. Immunol. 175:3309.
  14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
  15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
  16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
  17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
  18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
  19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
  20. Guo Y, et al. 2008. Blood 112:480. PubMed
  21. Kenna TJ, et al. 2008. Blood 111:2091.
  22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
  23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
  24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
  25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
  26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
  27. Qi Q, et al. 2009. Blood 114:564. PubMed
  28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
  29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
  32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations
  1. Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038/s41598-017-10135-1. PubMed
  2. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  3. Zhou L, et al. 2022. PLoS Negl Trop Dis. 16:e0010851. PubMed
  4. Ge Y, et al. 2022. iScience. 25:105437. PubMed
  5. Fan R, et al. 2023. J Immunother Cancer. 11: . PubMed
  6. Issa N, et al. 2023. Leukemia. :. PubMed
  7. Roberts JL, et al. 2022. Bone. 157:116310. PubMed
  8. Leonardi I, et al. 2022. Cell. 185:831. PubMed
  9. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  10. Wiedemann SJ, et al. 2022. Cell Metab. 34:991. PubMed
  11. Hao H, et al. 2023. Nat Biomed Eng. . PubMed
  12. Miao L, et al. 2020. Theranostics. 0.7625. PubMed
  13. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  14. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
  15. Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed
  16. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  17. Meng J, et al. 2011. Biol Reprod. 85:254. PubMed
  18. Wei H, et al. 2021. Malar J. 20:89. PubMed
  19. Earnest JT, et al. 2021. Cell Reports. 35(1):108962. PubMed
  20. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  21. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  22. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  23. Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
  24. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  25. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  26. Yang X, et al. 2019. Oncotarget. 10:4180. PubMed
  27. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  28. Maston LD, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 312:L609. PubMed
  29. Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed
  30. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  31. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  32. Kong YW, et al. 2020. Nat Commun. 3.322222222. PubMed
  33. Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed
  34. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  35. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  36. Wang W, et al. 2020. Immunity. 51(6):1102-1118.e7.. PubMed
  37. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  38. Ekeke CN, et al. 2020. J Thorac Cardiovasc Surg. S0022-5223:33337. PubMed
  39. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  40. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  41. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  42. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  43. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
  44. Shoji M, et al. 2012. Biochem Biophys Res Commun. 425:89. PubMed
  45. Kim S, et al. 2021. Elife. 10:. PubMed
  46. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  47. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  48. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  49. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  50. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  51. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  52. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  53. Yuan M, et al. 2022. Oxid Med Cell Longev. 2022:5479491. PubMed
  54. Fujii T, et al. 2021. Bone Res. 9:4. PubMed
  55. Kim M, et al. 2018. Immunity. 49:151. PubMed
  56. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  57. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  58. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  59. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
  60. Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed
  61. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
  62. Brown ZJ, et al. 2018. Cell Death Dis. 0.805555556. PubMed
  63. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  64. Zhong M, et al. 2022. Cell Death Dis. 13:671. PubMed
  65. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
  66. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  67. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  68. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
  69. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  70. Campisi L, et al. 2016. Nat Immunol. 10.1038/ni.3512. PubMed
  71. Bonaccorsi-Riani E, et al. 2015. PLoS One. 10: 0136106. PubMed
  72. Ge Y, et al. 2020. Mucosal Immunol. 13:34. PubMed
  73. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
RRID
AB_1877170 (BioLegend Cat. No. 100329)
AB_1877170 (BioLegend Cat. No. 100330)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account