- Clone
- 93 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Fcγ R III/II, Ly-17
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
101319 | 50 µg | ¥9,620 | |
101320 | 500 µg | ¥40,770 |
CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ at 1.0 µg per 106 cells in 100 µl volume for 5-10 minutes on ice prior to immunostaining. It is not necessary to wash cells between these blocking and immunostaining steps.
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Application References
(PubMed link indicates BioLegend citation) -
- Oliver AM, et al. 1999. Hybridoma 18:113.
- Brummel R and Lenert P. 2005. J. Immunol. 174:2429. PubMed
- Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349.
- Clements JL, et al. 2006. J. Immunol. 177:905. PubMed
- Flores M, et al. 2008. FASEB J. 22:3661. PubMed
- Ge XN, et al. 2010. J. Immunol. 185:1205. PubMed
- Maseda D, et al. 2012. J. Immunol. 188:1036. PubMed
- Lewis ND, et al. 2013. J. Immunol. 190:3533. PubMed
- Bonne-Annee S, et al. 2013. Infect Immun. 81:3346. PubMed
- Parlane NA, et al. 2013. Vet Immunol Immunopathol. 30:122. PubMed
- Jarajapu YP, et al. 2014. PLoS One. 9:93965. PubMed
- Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed
- Resa-Infante P, et al. 2014. J Virol. 88:8166. PubMed
- Gillis TP, et al. 2014. Infect Immun. 82:3900. PubMed
- Minkah N, et al. 2015. J Virol. 89:3366. PubMed
- Product Citations
-
- RRID
-
AB_1574973 (BioLegend Cat. No. 101319)
AB_1574973 (BioLegend Cat. No. 101320)
Antigen Details
- Structure
- Ig superfamily, 40-60 kD
- Distribution
-
B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
- Function
- Low affinity receptors for IgG
- Ligand/Receptor
- IgG
- Cell Type
- B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Fc Receptors
- Gene ID
- 14130 View all products for this Gene ID 14131 View all products for this Gene ID
- UniProt
- View information about CD16/32 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD16/32 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse CD16/32 | 93 | FC |
FITC anti-mouse CD16/32 | 93 | FC |
PE anti-mouse CD16/32 | 93 | FC |
Purified anti-mouse CD16/32 | 93 | FC,IP,Block |
Ultra-LEAF™ Purified anti-mouse CD16/32 | 93 | FC,IP,Block |
Alexa Fluor® 647 anti-mouse CD16/32 | 93 | FC |
PE/Cyanine7 anti-mouse CD16/32 | 93 | FC |
TruStain FcX™ (anti-mouse CD16/32) | 93 | FC |
PerCP/Cyanine5.5 anti-mouse CD16/32 | 93 | FC |
APC anti-mouse CD16/32 | 93 | FC |
APC/Cyanine7 anti-mouse CD16/32 | 93 | FC |
Brilliant Violet 421™ anti-mouse CD16/32 | 93 | FC |
Brilliant Violet 510™ anti-mouse CD16/32 | 93 | FC |
Purified anti-mouse CD16/32 (Maxpar® Ready) | 93 | FC,CyTOF® |
Brilliant Violet 711™ anti-mouse CD16/32 | 93 | FC |
TotalSeq™-A0109 anti-mouse CD16/32 | 93 | PG |
TotalSeq™-B0109 anti-mouse CD16/32 | 93 | PG |
TotalSeq™-C0109 anti-mouse CD16/32 | 93 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse CD16/32
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FITC anti-mouse CD16/32
-
PE anti-mouse CD16/32
-
Purified anti-mouse CD16/32
-
Ultra-LEAF™ Purified anti-mouse CD16/32
-
Alexa Fluor® 647 anti-mouse CD16/32
-
PE/Cyanine7 anti-mouse CD16/32
-
TruStain FcX™ (anti-mouse CD16/32)
-
PerCP/Cyanine5.5 anti-mouse CD16/32
-
APC anti-mouse CD16/32
-
APC/Cyanine7 anti-mouse CD16/32
-
Brilliant Violet 421™ anti-mouse CD16/32
-
Brilliant Violet 510™ anti-mouse CD16/32
-
Purified anti-mouse CD16/32 (Maxpar® Ready)
-
Brilliant Violet 711™ anti-mouse CD16/32
-
TotalSeq™-A0109 anti-mouse CD16/32
-
TotalSeq™-B0109 anti-mouse CD16/32
-
TotalSeq™-C0109 anti-mouse CD16/32
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