- Clone
- 5E8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CC CKR3, MIP1-alpha receptor like-2, eotaxin receptor, CD193, CCR3
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD193, also known as CC-chemokine receptor 3 (CCR3), CC CKR3, MIP1-alpha receptor like-2, and eotaxin receptor, is a member of the G protein-coupled seven transmembrane receptors family. It binds to the CC chemokines eotaxin, eotaxin-2, and eotaxin-3 with high affinity. CCR3 has also been reported to bind RANTES, MCP-3, and MCP-4 with low affinity. CCR3 receptor is expressed on human eosinophils, basophils, mast cells, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitor cells, Th2-like lymphocytes, and keratinocytes. CCR3 is thought to play a role in allergic diseases such as bronchial asthma and allergic rhinitis. CCR3 is a co-receptor for HIV-1 and HIV-2, and the binding of eotaxin with CCR3 has been shown to inhibit HIV infection in some cell types.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: The 5E8 antibody is useful for immunofluorescent staining and flow cytometric analysis of CCR3 expression.
It has been observed that the 5E8 antibody clone can interact with PE/Cyanine7 antibody conjugates during multi-color staining, potentially leading to unwanted staining. This interaction can be resolved by sequentially staining with the 5E8 antibody first and then followed by the PE/Cyanine7 conjugate of interest. -
Application References
(PubMed link indicates BioLegend citation) -
- Beauvillian C, et al. 2011. Blood 117:1196. PubMed
- RRID
-
AB_2650970 (BioLegend Cat. No. 310726)
Antigen Details
- Structure
- G-protein coupled seven transmembrane domain receptor, 356 amino acids
- Distribution
-
Eosinophils, basophils, mast, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitor, Th2, keratinocytes
- Function
- Co-receptor for HIV-1 and HIV-2, allergy
- Receptors
- Eotaxin, eotaxin-2, eotaxin-3
- Cell Type
- Eosinophils, Erythrocytes, Hematopoietic stem and progenitors, Macrophages, Mast cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Gerard W, et al. 1996. J. Exp. Med. 183:2437.
2. Uguccioni C, et al. 1997. J. Clin. Invest. 100:1137.
3. Sallusto F, et al. 1997. Science. 277:2005.
4. Loetscher P, et al. 2001. J. Biol. Chem. 276:2986. - Regulation
- Upregulated by high affinity Fc IgE receptor ligation (mast cells), RANTES (keratinocytes), IFNg (monocytes, neutrophils), HIV tat protein (basophils), IL-3, IL-5 and GM-CSF (CD34+ progenitor cells), IL-2 and IL-4(T cells). Downregulated by IL-
- Gene ID
- 1232 View all products for this Gene ID
- UniProt
- View information about CD193 on UniProt.org
Related FAQs
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
-
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
Other Formats
View All CD193 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD193 (CCR3)
Human peripheral blood granulocytes stained with 5E8 PE and ... Human paraffin-embedded skin tissue slices were prepared wit... -
PE anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 APC... -
Brilliant Violet 605™ anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 APC... -
APC anti-human CD193 (CCR3)
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Alexa Fluor® 647 anti-human CD193 (CCR3)
Human peripheral blood granulocytes stained with CD16 FITC a... -
APC/Cyanine7 anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 FIT... -
Brilliant Violet 421™ anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 FIT... -
PerCP/Cyanine5.5 anti-human CD193 (CCR3)
Human peripheral blood granulocytes were stained with CD16 A... -
FITC anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 APC... -
Brilliant Violet 510™ anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 FIT... -
Biotin anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 APC... -
APC/Fire™ 750 anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 FIT... -
PE/Dazzle™ 594 anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 FIT... -
TotalSeq™-A0397 anti-human CD193 (CCR3)
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TotalSeq™-C0397 anti-human CD193 (CCR3)
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Brilliant Violet 711™ anti-human CD193 (CCR3) Antibody
Human peripheral blood leukocytes were stained with CD16 APC... -
TotalSeq™-D0397 anti-human CD193 (CCR3)
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TotalSeq™-B0397 anti-human CD193 (CCR3)
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Spark Blue™ 550 anti-human CD193 (CCR3) (Flexi-Fluor™)
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Spark Red™ 718 anti-human CD193 (CCR3) (Flexi-Fluor™)
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