APCFire 750 anti-human CD147 Antibody, CD147, HIM6

Clone: HIM6 (See other available formats)
Regulatory Status: RUO
Workshop: VI N-L109
Other Names: Neurothelin, Basigin, EMMPRIN
Isotype: Mouse IgG1, κ
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Product Citations: publications

HIM6_APCFire750_CD147_Antibody_073118 — Human peripheral blood lymphocytes were stained with CD147 (clone HIM6, filled histogram) APC/Fire™ 750 or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).

Compare all formats See APC/Fire™ 750 spectral data

Cat #	Size	Price	Save
306217	25 tests	¥31,020
306218	100 tests	¥63,360

Description

CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65 kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Baboon, Capuchin Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PBMCs
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: inhibition of T cell activation², immunohistochemical staining^1,3 of frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, and Western blotting¹. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306221 and 306222).
RRID: AB_2750173 (BioLegend Cat. No. 306217)
AB_2750173 (BioLegend Cat. No. 306218)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 55-65 kD
Distribution: Leukocytes, erythrocytes, platelets, endothelial cells
Function: Adhesion
Cell Type: Endothelial cells, Erythrocytes, Leukocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Biswas C, et al. 1995. Cancer Res. 55:434.
2. Fadool J, et al. 1993. Dev. Dyn. 196:252.
3. Felzmann T, et al. 1991. J. Clin. Immunol. 11:205.
Gene ID: 682 View all products for this Gene ID
UniProt: View information about CD147 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD147 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-human CD147	HIM6	FC
Purified anti-human CD147	HIM6	FC,IHC-P
Alexa Fluor® 488 anti-human CD147	HIM6	FC,ICC
Alexa Fluor® 647 anti-human CD147	HIM6	FC,ICC,IHC-P
PE anti-human CD147	HIM6	FC
APC/Fire™ 750 anti-human CD147	HIM6	FC
APC anti-human CD147	HIM6	FC
PE/Cyanine7 anti-human CD147	HIM6	FC
PerCP/Cyanine5.5 anti-human CD147	HIM6	FC
Ultra-LEAF™ Purified anti-human CD147	HIM6	FC,WB,IHC,Block
TotalSeq™-C1137 anti-human CD147	HIM6	PG
Brilliant Violet 605™ anti-human CD147	HIM6	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07/31/2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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