Ultra-LEAF™ Purified anti-human CD147 Antibody, CD147, HIM6

Clone: HIM6 (See other available formats)
Regulatory Status: RUO
Workshop: VI N-L109
Other Names: Neurothelin, Basigin, EMMPRIN
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

Human peripheral blood lymphocytes stained with Ultra-LEAF™ purified HIM6, followed by anti-mouse IgG FITC

Cat #	Size	Price	Save
306221	100 µg	¥44,820
306222	1 mg	¥123,340

Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65 kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Baboon, Capuchin Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PBMCs
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
WB, IHC, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: inhibition of T cell activation², immunohistochemical staining^1,3 of frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, and Western blotting¹. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306221 and 306222).
RRID: AB_2860815 (BioLegend Cat. No. 306221)
AB_2860815 (BioLegend Cat. No. 306222)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 55-65 kD
Distribution: Leukocytes, erythrocytes, platelets, endothelial cells
Function: Adhesion
Cell Type: Endothelial cells, Erythrocytes, Leukocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Biswas C, et al. 1995. Cancer Res. 55:434.
2. Fadool J, et al. 1993. Dev. Dyn. 196:252.
3. Felzmann T, et al. 1991. J. Clin. Immunol. 11:205.
Gene ID: 682 View all products for this Gene ID
UniProt: View information about CD147 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD147 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-human CD147	HIM6	FC
Purified anti-human CD147	HIM6	FC,IHC-P
Alexa Fluor® 488 anti-human CD147	HIM6	FC,ICC
Alexa Fluor® 647 anti-human CD147	HIM6	FC,ICC,IHC-P
PE anti-human CD147	HIM6	FC
APC/Fire™ 750 anti-human CD147	HIM6	FC
APC anti-human CD147	HIM6	FC
PE/Cyanine7 anti-human CD147	HIM6	FC
PerCP/Cyanine5.5 anti-human CD147	HIM6	FC
Ultra-LEAF™ Purified anti-human CD147	HIM6	FC,WB,IHC,Block
TotalSeq™-C1137 anti-human CD147	HIM6	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03/24/2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.