Biotin anti-Neurofilament H & M NF-H/NF-M, Phosphorylated Antibody anti-Neurofilament H & M (NF-H/NF-M), Phospho

Clone: SMI 310 (See other available formats)
Regulatory Status: RUO
Other Names: Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Isotype: Mouse IgG1, κ
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SMI31_Biotin_Neurofilament_Phospho_Antibody_WB_011717 — Western blot of anti-Neurofilament H & M (NF-H/NF-M), Phosphorylated antibody (clone SMI 310) conjugated to biotin. Lane 1: 20 µg of rat brain lysate; Lane 2: 20 µg of mouse brain lysate. The blots were incubated with the biotin conjugated antibody or IgG1 control at 5 µg/mL for two hours at room temperature, followed by one hour incubation with HRP Streptavidin secondary antibody. Enhanced chemiluminescence was used as the detection system.

Cat #	Size	Price	Save
837706	100 µg	¥66,000

Description

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and regulate axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE: the light or lowest (NF-L) runs at 68-70 kD; the medium or middle (NF-M) runs at about 145-160 kD; the heavy or highest (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from glia (negative for NF).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting⁴, immunocytochemistry² and immunohistochemical staining of frozen tissue sections^{1, 3}.

Clone SMI 310 reacts with an extensively phosphorylated epitope of neurofilament H and, to a much lesser extent, neurofilament M in most mammalian species. Phosphatase treatment of samples abolishes reaction with SMI 310. A very extensive degree of hyperphosphorylation of neurofilaments seems to be necessary for its reactivity.
RRID: AB_2650682 (BioLegend Cat. No. 837706)

Antigen Details

Structure: The medium or middle neurofilament (NF-M) runs at 145-160 kD, and the heavy or highest neurofilament (NF-H) runs at 200-220 kD.
Distribution: Tissue distribution: CNS, peripheral nerves and glandular cells of the prostate.
Cellular distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion.
Function: Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.
Interaction: Cell bodies and dendrites are generally unstained while other cells and tissues are unreactive, except for peripheral axons.
Cell Type: Mature Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments, Phospho-Proteins
Antigen References: Siedler D, et al. 2014. Front Cell Neurosci. 8:429.
Gene ID: 4744 View all products for this Gene ID
UniProt: View information about Neurofilament HM NF-H NF-M Phospho on UniProt.org

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Western Blotting Protocol

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Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Neurofilament H&M (NF-H/NF-M), Phospho Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Neurofilament H & M (NF-H/NF-M), Phosphorylated	SMI 310	IHC-P,WB,IHC-F,ICC
Biotin anti-Neurofilament H & M (NF-H/NF-M), Phosphorylated	SMI 310	WB
Alexa Fluor® 647 anti-Neurofilament H & M (NF-H/NF-M), Phosphorylated	SMI 310	IHC-P
Alexa Fluor® 488 anti-Neurofilament H & M (NF-H/NF-M), Phosphorylated	SMI 310	IHC-P

Certificate of Analysis

Go To Top Version: 2 Revision Date: 01/31/2018

For Research Use Only. Not for diagnostic or therapeutic use.

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