Brilliant Violet 421™ anti-mouse CD11a Antibody, CD11a, 2D7

Clone: 2D7 (See other available formats)
Regulatory Status: RUO
Other Names: αL integrin, LFA-1 α subunit, Ly-15, Ly-21, ITGAL
Isotype: Rat IgG2a, κ
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Product Citations: publications

2D7_BV-421_CD11a_FC_062424 — C57BL/6 splenocytes were stained with anti-mouse CD11a (clone 2D7) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).

Compare all formats See Brilliant Violet 421™ spectral data

Cat #	Size	Price	Save
162909	50 µg	¥59,400

Description

CD11a is a 180 kD glycoprotein, also known as α_L integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)

Antigen Details

Structure: Integrin, associated with integrin CD18, 180 kD
Distribution: Lymphocytes, granulocytes, monocytes, macrophages
Function: Cellular adhesion
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Cell Type: Granulocytes, Lymphocytes, Macrophages, Monocytes
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1. .Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479.
Gene ID: 16408 View all products for this Gene ID
UniProt: View information about CD11a on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD11a Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD11a Antibody	2D7	FC,IHC-F
Alexa Fluor® 488 anti-mouse CD11a	2D7	FC
APC/Fire™ 750 anti-mouse CD11a	2D7	FC
PerCP/Cyanine5.5 anti-mouse CD11a	2D7	FC
Brilliant Violet 421™ anti-mouse CD11a	2D7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/24/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.