- Clone
- E18 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tp44, T44
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The E18 antibody blocks the binding of 37.51 antibody to CD28 and costimulates T cell activation and proliferation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- A20 cells and recombinant mCD28Ig
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, and in vitro induction of thymocyte differentiation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 122004).
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Application References
(PubMed link indicates BioLegend citation) -
- Dennehy KM, et al. 2006. J. Immunol. 176:5725.
Antigen Details
- Structure
- Ig superfamily, 44 kD
- Distribution
-
Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
- Function
- Costimulates T and NK cells
- Ligand/Receptor
- CD80 (B7-1), CD86 (B7-2)
- Cell Type
- NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380. - Gene ID
- 12487 View all products for this Gene ID
- UniProt
- View information about CD28 on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD28 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD28 | E18 | FC |
FITC anti-mouse CD28 | E18 | FC |
PE anti-mouse CD28 | E18 | FC |
PE/Cyanine7 anti-mouse CD28 | E18 | FC |
APC anti-mouse CD28 | E18 | FC |
Alexa Fluor® 488 anti-mouse CD28 | E18 | FC |
Ultra-LEAF™ Purified anti-mouse CD28 | E18 | FC,Block,Costim |
Brilliant Violet 421™ anti-mouse CD28 | E18 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD28
C57BL/6 mouse splenocytes stained with purified E18, followe... -
FITC anti-mouse CD28
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) PE and... -
PE anti-mouse CD28
C57BL/6 mouse splenocytes stained with E18 PE -
PE/Cyanine7 anti-mouse CD28
C57BL/6 mouse thymocytes stained with E18 PE/Cyanine7 -
APC anti-mouse CD28
C57BL/6 mouse thymocytes stained with E18 APC -
Alexa Fluor® 488 anti-mouse CD28
C57BL/6 mouse splenocytes stained with E18 Alexa Fluor® ... -
Ultra-LEAF™ Purified anti-mouse CD28
C57BL/6 mouse splenocytes stained with LEAF™ E18, followed b... -
Brilliant Violet 421™ anti-mouse CD28
C57BL/6 splenocytes were stained with anti-mouse CD3 (clone ...
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