- Clone
- E18 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tp44, T44
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
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122021 | 100 µg | ¥25,570 | |
122022 | 1 mg | ¥88,650 |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The E18 antibody blocks the binding of 37.51 antibody to CD28 and costimulates T cell activation and proliferation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- A20 cells and recombinant mCD28Ig
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Costim, Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, and in vitro induction of thymocyte differentiation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 122004).
-
Application References
(PubMed link indicates BioLegend citation) -
- Dennehy KM, et al. 2006. J. Immunol. 176:5725.
- Product Citations
-
- RRID
-
AB_2810370 (BioLegend Cat. No. 122021)
AB_2810370 (BioLegend Cat. No. 122022)
Antigen Details
- Structure
- Ig superfamily, 44 kD
- Distribution
-
Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
- Function
- Costimulates T and NK cells
- Ligand/Receptor
- CD80 (B7-1), CD86 (B7-2)
- Cell Type
- NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380. - Gene ID
- 12487 View all products for this Gene ID
- UniProt
- View information about CD28 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD28 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD28 | E18 | FC |
FITC anti-mouse CD28 | E18 | FC |
PE anti-mouse CD28 | E18 | FC |
PE/Cyanine7 anti-mouse CD28 | E18 | FC |
APC anti-mouse CD28 | E18 | FC |
Alexa Fluor® 488 anti-mouse CD28 | E18 | FC |
Ultra-LEAF™ Purified anti-mouse CD28 | E18 | FC,Block,Costim |
Brilliant Violet 421™ anti-mouse CD28 | E18 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD28
C57BL/6 mouse splenocytes stained with purified E18, followe... -
FITC anti-mouse CD28
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) PE and... -
PE anti-mouse CD28
C57BL/6 mouse splenocytes stained with E18 PE -
PE/Cyanine7 anti-mouse CD28
C57BL/6 mouse thymocytes stained with E18 PE/Cyanine7 -
APC anti-mouse CD28
C57BL/6 mouse thymocytes stained with E18 APC -
Alexa Fluor® 488 anti-mouse CD28
C57BL/6 mouse splenocytes stained with E18 Alexa Fluor® ... -
Ultra-LEAF™ Purified anti-mouse CD28
C57BL/6 mouse splenocytes stained with LEAF™ E18, followed b... -
Brilliant Violet 421™ anti-mouse CD28
C57BL/6 splenocytes were stained with anti-mouse CD3 (clone ...
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