Brilliant Violet 605™ anti-human TCR Vα24-Jα18 (iNKT cell), TCR Vα24-Jα18, 6B11

Clone: 6B11 (See other available formats)
Regulatory Status: RUO
Other Names: TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT
Isotype: Mouse IgG1, κ
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Product Citations: publications

6B11_BV605_TCR_Valpha24-Jalpha18_Antibody_082919 — Human peripheral blood lymphocytes were stained with CD3 FITC and anti-human TCR Vα24-Jα18 (iNKT cell) (clone 6B11) Brilliant Violet 605™ (left) or mouse IgG1, κ Brilliant Violet 605™ isotype control (right).

Compare all formats See Brilliant Violet 605™ spectral data

Cat #	Size	Price	Save
342929	25 tests	¥44,880
342930	100 tests	¥95,700

Description

Encoded by the TCR Vα24-Jα18 germline configuration, Vα24-JαQ is expressed on a subset of NKT cells, namely invariant NKT (iNKT). Vα24-JαQ TCR interacts with the glycolipid loaded MHC class 1b molecule CD1d, inducing activation and subsequent cytokine production. iNKT cells have been implicated in immune regulation, tumor surveillance, and host response to pathogens. While iNKT cells occur at low frequency in the blood, assorted chemokines contribute to their tissue homing potential.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Rhesus, Pigtailed Macaque, Sooty Mangabey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The 6B11 antibody recognizes the invariant CDR3 region of TCR Va24-JaQ.
RRID: AB_2814261 (BioLegend Cat. No. 342929)
AB_2814261 (BioLegend Cat. No. 342930)

Antigen Details

Structure: Invariant CDR3 region of TCR Vα24-JαQ
Distribution: Invariant NKT cells
Ligand/Receptor: Glycolipid loaded CD1d
Cell Type: NKT cells
Biology Area: Immunology
Molecular Family: TCRs
Antigen References: Thomas SY, et al. 2003. J. Immunol. 171:2571.

Exley MA, et al. 2008. Eur. J. Immunol. 38:1756.

Montoya CJ, et al. 2007. Immunology. 122:1.

Gansuvd B, et al. 2003. J. Immunol. 171:2904.
Gene ID: 28659 View all products for this Gene ID
UniProt: View information about TCR Valpha24-Jalpha18 on UniProt.org

Documentation

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Other Formats

View All TCR Vα24-Jα18 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
PE anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
FITC anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
APC anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
PE/Cyanine7 anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
PerCP/Cyanine5.5 anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
Brilliant Violet 421™ anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
Brilliant Violet 510™ anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
PE/Dazzle™ 594 anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
Brilliant Violet 711™ anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
TotalSeq™-A0584 anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	PG
TotalSeq™-C0584 anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	PG
Brilliant Violet 605™ anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
Brilliant Violet 785™ anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
TotalSeq™-B0584 anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	PG
APC/Fire™ 750 anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
APC/Cyanine7 anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
PerCP/Fire™ 780 anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
PerCP/Fire™ 806 anti-human TCR Vα24-Jα18 (iNKT cell)	6B11	FC
Spark Red™ 718 anti-human TCR Vα24-Jα18 (Flexi-Fluor™)	6B11	FC
Spark Blue™ 574 anti-human TCR Vα24-Jα18 (Flexi-Fluor™)	6B11	FC
Spark Blue™ 550 anti-human TCR Vα24-Jα18 (Flexi-Fluor™)	6B11	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 12/16/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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