Brilliant Violet 650™ anti-human CD64 Antibody, CD64, 10.1

Clone: 10.1 (See other available formats)
Regulatory Status: RUO
Workshop: VI MA36
Other Names: FcγRI, FcR I
Isotype: Mouse IgG1, κ
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10pt1_CD64_BV650_Antibody_021323 — Human peripheral blood monocytes were stained with anti-human CD64 (clone 10.1) Brilliant Violet 650™ (filled histogram) or mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram).

Compare all formats See Brilliant Violet 650™ spectral data

Cat #	Size	Price	Save
305053	25 tests	¥48,620
305054	100 tests	¥95,700

Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Baboon, Capuchin Monkey, Chimpanzee, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI^2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections¹².
RRID: AB_2936742 (BioLegend Cat. No. 305053)
AB_2936742 (BioLegend Cat. No. 305054)

Antigen Details

Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, dendritic cells, activated granulocytes
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG receptor
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.
Gene ID: 2209 View all products for this Gene ID
UniProt: View information about CD64 on UniProt.org

Documentation

Reviews

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Other Formats

View All CD64 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-human CD64	10.1	FC
FITC anti-human CD64	10.1	FC
PE anti-human CD64	10.1	FC
Purified anti-human CD64	10.1	FC,IHC-F,Block
Alexa Fluor® 488 anti-human CD64	10.1	FC
Alexa Fluor® 647 anti-human CD64	10.1	FC
APC anti-human CD64	10.1	FC
Pacific Blue™ anti-human CD64	10.1	FC
Brilliant Violet 421™ anti-human CD64	10.1	FC
PE/Cyanine7 anti-human CD64	10.1	FC
PerCP/Cyanine5.5 anti-human CD64	10.1	FC
APC/Cyanine7 anti-human CD64	10.1	FC
Brilliant Violet 510™ anti-human CD64	10.1	FC
Purified anti-human CD64 (Maxpar® Ready)	10.1	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD64	10.1	FC
Brilliant Violet 605™ anti-human CD64	10.1	FC
APC/Fire™ 750 anti-human CD64	10.1	FC
PE anti-human CD64	10.1	FC
PE/Dazzle™ 594 anti-human CD64	10.1	FC
FITC anti-human CD64	10.1	FC
TotalSeq™-A0162 anti-human CD64	10.1	PG
Brilliant Violet 711™ anti-human CD64	10.1	FC
Alexa Fluor® 700 anti-human CD64	10.1	FC
Brilliant Violet 785™ anti-human CD64	10.1	FC
TotalSeq™-C0162 anti-human CD64	10.1	PG
Ultra-LEAF™ Purified anti-human CD64	10.1	FC,IHC-F,Block
TotalSeq™-B0162 anti-human CD64	10.1	PG
TotalSeq™-D0162 anti-human CD64	10.1	PG
GMP PE anti-human CD64	10.1	FC
GMP FITC anti-human CD64	10.1	FC
Brilliant Violet 650™ anti-human CD64	10.1	FC
GMP PE/Dazzle™ 594 anti-human CD64	10.1	FC
PE/Cyanine7 anti-human CD64	10.1	FC
APC/Fire™ 750 anti-human CD64	10.1	FC
PerCP/Cyanine5.5 anti-human CD64	10.1	FC
Pacific Blue™ anti-human CD64	10.1	FC
GMP APC/Fire™ 750 anti-human CD64	10.1	FC
GMP PE/Cyanine7 anti-human CD64	10.1	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 02/13/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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