Brilliant Violet 650™ anti-mouse CD326 (Ep-CAM) Antibody, CD326, G8.8

Clone: G8.8 (See other available formats)
Regulatory Status: RUO
Other Names: CD326, EGP40, MIC18, TROP1, KSA
Isotype: Rat IgG2a, κ
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Product Citations: publications

G8-8_BV650_CD326_Antibody_081120 — TE-71 (mouse thymic epithelial stromal cell line) was stained with CD326 (clone G8.8) Brilliant Violet 650™ (filled histogram) or rat IgG2a, κ Brilliant Violet 650™ isotype control (open histogram).

Compare all formats See Brilliant Violet 650™ spectral data

Cat #	Size	Price	Save
118241	50 µg	¥65,340

Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: TE-71 thymic epithelial cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed¹, with or without OCT embedding^2,4, and spatial biology (IBEX)^13,14.
RRID: AB_2876432 (BioLegend Cat. No. 118241)

Antigen Details

Structure: 40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution: Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.
Function: Mediates calcium-independent homophilic cell-cell adhesion.
Interaction: CD326 displays hemophilic binding.
Ligand/Receptor: CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type: Embryonic Stem Cells, Epithelial cells
Biology Area: Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.
Gene ID: 17075 View all products for this Gene ID
UniProt: View information about CD326 on UniProt.org

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD326 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD326 (Ep-CAM)	G8.8	FC
Purified anti-mouse CD326 (Ep-CAM)	G8.8	FC,IHC-F,ICC
Biotin anti-mouse CD326 (Ep-CAM)	G8.8	FC
PE anti-mouse CD326 (Ep-CAM)	G8.8	FC
FITC anti-mouse CD326 (Ep-CAM)	G8.8	FC
Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM)	G8.8	FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM)	G8.8	FC,IHC-F,3D IHC,SB
PE/Cyanine7 anti-mouse CD326 (Ep-CAM)	G8.8	FC
APC/Cyanine7 anti-mouse CD326 (Ep-CAM)	G8.8	FC
PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM)	G8.8	FC
Alexa Fluor® 594 anti-mouse CD326 (Ep-CAM)	G8.8	ICC,IHC-F,3D IHC
Brilliant Violet 421™ anti-mouse CD326 (Ep-CAM)	G8.8	FC
Brilliant Violet 605™ anti-mouse CD326 (Ep-CAM)	G8.8	FC
Purified anti-mouse CD326 (Ep-CAM) (Maxpar® Ready)	G8.8	FC,CyTOF®
APC/Fire™ 750 anti-mouse CD326 (Ep-CAM)	G8.8	FC
Brilliant Violet 711™ anti-mouse CD326 (Ep-CAM)	G8.8	FC
Brilliant Violet 510™ anti-mouse CD326 (Ep-CAM)	G8.8	FC
PE/Dazzle™ 594 anti-mouse CD326 (Ep-CAM)	G8.8	FC
TotalSeq™-A0449 anti-mouse CD326 (Ep-CAM)	G8.8	PG
Alexa Fluor® 700 anti-mouse CD326 (Ep-CAM)	G8.8	FC
TotalSeq™-C0449 anti-mouse CD326 (Ep-CAM)	G8.8	PG
Brilliant Violet 785™ anti-mouse CD326 (Ep-CAM)	G8.8	FC
TotalSeq™-B0449 anti-mouse CD326 (Ep-CAM)	G8.8	PG
Brilliant Violet 650™ anti-mouse CD326 (Ep-CAM)	G8.8	FC
PE/Cyanine5 anti-mouse CD326 (Ep-CAM)	G8.8	FC
Spark Red™ 718 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™)	G8.8	FC
Spark Blue™ 574 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™)	G8.8	FC
Spark Blue™ 550 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™)	G8.8	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 08/11/2020

For Research Use Only. Not for diagnostic or therapeutic use.

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