FITC anti-human CD18 Antibody anti-CD18

Clone: CBR LFA-1/2 (See other available formats)
Regulatory Status: RUO
Other Names: Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2, b2 integrin, beta 2 integrin
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

CBRspaceLFA-1slash2_FITC_CD18_Antibody_FC_031915 — Human peripheral blood lymphocytes were stained with CD18 (clone CBR LFA-1/2) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).

Compare all formats See FITC spectral data

Cat #	Size	Price	Save
366305	25 tests	¥22,220
366306	100 tests	¥53,020

Description

CD18, also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin, is a 90 - 95 kD type I glycoprotein. CD18 non-covalently associates with CD11a, CD11b, or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in the adhesion and signaling in hematopoietic cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human LFA-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats of this clone) include: activation of LFA-1 and MAC-1^1,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for function assays (contact our custom solutions team).
RRID: AB_2565529 (BioLegend Cat. No. 366305)
AB_2565529 (BioLegend Cat. No. 366306)

Antigen Details

Structure: 90 - 95 kD type I transmembrane glycoprotein.
Distribution: Leukocytes.
Function: Cell adhesion and signaling.
Interaction: Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c
Ligand/Receptor: ICAMs.
Cell Type: Leukocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer TA. 1994. Cell 76:301.
Gene ID: 3689 View all products for this Gene ID
UniProt: View information about CD18 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

Other Formats

View All CD18 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD18	CBR LFA-1/2	FC
PE anti-human CD18	CBR LFA-1/2	FC
APC anti-human CD18	CBR LFA-1/2	FC
FITC anti-human CD18	CBR LFA-1/2	FC
PE/Cyanine7 anti-human CD18	CBR LFA-1/2	FC
Alexa Fluor® 647 anti-human CD18	CBR LFA-1/2	FC
APC/Fire™ 750 anti-human CD18	CBR LFA-1/2	FC
PerCP/Cyanine5.5 anti-human CD18	CBR LFA-1/2	FC

Certificate of Analysis

Customers Also Purchased

PE anti-human CD49d

Clone: 9F10

APC anti-human CD62E

Clone: HAE-1f

FITC anti-human CD62P (P-Selectin)

Clone: AK4

Ultra-LEAF™ Purified anti-mouse CD54

Clone: YN1/1.7.4

Go To Top Version: 1 Revision Date: 03/19/2015

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.