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FITC anti-mouse Qa-2 Antibody

Pricing & Availability
Clone
695H1-9-9 (See other available formats)
Regulatory Status
RUO
Other Names
Nonclassical MHC-Ib, H2-Qa2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
695H1-9-9_FITC_Qa-2_Antibody_FC_041714
C57BL/6 mouse splenocytes were stained with Qa-2 (clone 695H1-9-9) FITC (filled histogram) or mouse IgG2a FITC isotype control (open histogram).
  • 695H1-9-9_FITC_Qa-2_Antibody_FC_041714
    C57BL/6 mouse splenocytes were stained with Qa-2 (clone 695H1-9-9) FITC (filled histogram) or mouse IgG2a FITC isotype control (open histogram).
Compare all formats See FITC spectral data
Cat # Size Price Save
121709 50 µg ¥18,260
121710 500 µg ¥69,740
Description

Mouse Qa-2 is a 40 kD GPI-linked surface protein. It is a member of nonclassical MHC class Ib molecule encoded by Q5, Q6, Q7, Q9, and Q10 genes of the H-2 complex. Qa-2 is a nonpolymorphic molecule primarily expressed on mouse T and B cells. The expression level of Qa-2 on mature T cells is higher than on B cells. Different mouse strains express Qa-2 at varying levels. Qa-2, in association with β2-microglobulin, binds to peptide and plays a role in antigen presentation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H.SW mouse skin graft and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2.

Application References

(PubMed link indicates BioLegend citation)
  1. Sharrow SO, et al. 1989. J. Immunol. 142:3495.
  2. Patk E, et al. 1993. Microbiol. Immunol. 37:743.
Product Citations
  1. Mardomi A, et al. 2021. J Cell Physiol. 236:6328. PubMed
  2. Solanki A, et al. 2018. Development. 145. PubMed
  3. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  4. Liang Z, et al. 2017. Autophagy. 14:505. PubMed
RRID
AB_2563434 (BioLegend Cat. No. 121709)
AB_2563434 (BioLegend Cat. No. 121710)

Antigen Details

Structure
GPI-linked protein, associates with β2-microglobulin, 40 kD
Distribution

T and B cells, mature T cells expression higher than B cells

Function
Antigen presentation, promote immune response
Ligand/Receptor
Peptide
Cell Type
B cells, T cells
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Sharrow SO, et al. 1989. J. Immunol. 142:3495.
2. Mann DW, et al. 1987. J. Immunol. 138:240.
3. Mellor AL, et al.1985. Proc. Natl. Acad. Sci.USA. 82:5920.
4. Tabaczewski P, et al. 1997. J. Immunol. 159:2771.
5. Chiang EY and Stroynowski I. 2005. J. Immunol. 174:5367.

Gene ID
111458 View all products for this Gene ID
UniProt
View information about Qa-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/24/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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