FITC anti-rat CD86 Antibody anti-CD86

Clone: 24F (See other available formats)
Regulatory Status: RUO
Other Names: B7-2, B70
Isotype: Mouse IgG1, κ
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24F_FITC_091907 — ConA-stimulated (3 days) LOU rat splenocytes stained with 24F FITC

Compare all formats See FITC spectral data

Cat #	Size	Price	Save
200305	50 µg	¥21,560

Description

CD86 is an 80 kD member of the immunoglobulin superfamily also known as B70 or B7-2. It is expressed on antigen presenting cells and activated T and B cells. CD86 is a ligand for CD28 and CD152 (CTLA-4). It is one of the accessory molecules that plays an important role in T cell-APC cell costimulatory interactions. The 24F antibody can block the co-stimulatory activity of CD86.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation^1-3, blocking of costimulation of T cells^1-3, and immunohistochemical staining of acetone-fixed frozen sections³. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200310).
RRID: AB_313854 (BioLegend Cat. No. 200305)

Antigen Details

Structure: Ig superfamily, 80 kD
Distribution: Antigen presenting cells, activated B cells and T cells
Function: T and B cell costimulation
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: B cells, T cells, Antigen-presenting cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Ann. Rev. Immunol. 20:29.
Gene ID: 56822 View all products for this Gene ID
UniProt: View information about CD86 on UniProt.org

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

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Other Formats

View All CD86 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-rat CD86	24F	FC
FITC anti-rat CD86	24F	FC
Biotin anti-rat CD86	24F	FC
Alexa Fluor® 647 anti-rat CD86	24F	FC
APC anti-rat CD86	24F	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

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