GMP PE anti-human CD103 (Integrin αE) Antibody, CD103, Ber-ACT8

Clone: Ber-ACT8 (See other available formats)
Workshop: V A067
Other Names: Integrin alpha E (ITGAE)
Isotype: Mouse IgG1, κ
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Product Citations: publications

Ber-ACT8_GMP_PE_CD103_111323 — Typical result of PHA-stimulated (3 days) human peripheral blood lymphocytes stained either with Ber-ACT8 PE used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

Compare all formats See GMP PE spectral data

Cat #	Size	Price	Save
260330	100 tests	¥67,100

Description

CD103 is a type I transmembrane glycoprotein also known as αE integrin, integrin αIEL chain, and human mucosal lymphocyte antigen 1. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. Treg cells express high level of CD103. Hairy cell leukemia has also been shown to express CD103. The expression of CD103 on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as an αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Product Details

Technical data sheet

Product Details

Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HTLV-1 induced human T cell line MAPS16
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 12 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western Blotting¹, immunoprecipitation¹, and immunohistochemical staining of frozen tissue sections¹.

Antigen Details

Structure: Type I transmembrane glycoprotein, integrin family; can be cleaved into 150 kD and 25 kD chains; associated with β7 integrin
Distribution: Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells; expressed on hairy cell leukemia
Function: Retention and activation of CD103⁺ lymphocytes in the intestinal epithelium, regulation of tissue-specific T cell homing
Ligand/Receptor: E-Cadherin
Cell Targets: Integrin β7
Cell Type: Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Parker CM, et al. 1992. P. Natl. Acad. Sci. USA 89:1924.
2. Kruschwitz M, et al. 1991. J. Clin. Pathol. 44:636.
3. Schon MP, et al. 1999. J. Immunol. 162:6641.
4. Shaw SK, et al. 1994. J. Biol. Chem. 269:6016.
Gene ID: 3682 View all products for this Gene ID
UniProt: View information about CD103 on UniProt.org

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What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

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Description	Clone	Applications
Purified anti-human CD103 (Integrin αE)	Ber-ACT8	FC,WB,IP,IHC-F
FITC anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Alexa Fluor® 488 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Alexa Fluor® 647 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE/Cyanine7 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Brilliant Violet 421™ anti-human CD103 (Integrin αE)	Ber-ACT8	FC
APC anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Brilliant Violet 605™ anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Biotin anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Brilliant Violet 711™ anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE/Dazzle™ 594 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PerCP/Cyanine5.5 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Brilliant Violet 785™ anti-human CD103 (Integrin αE)	Ber-ACT8	FC
APC/Cyanine7 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
TotalSeq™-A0145 anti-human CD103 (Integrin αE)	Ber-ACT8	PG
TotalSeq™-C0145 anti-human CD103 (Integrin αE)	Ber-ACT8	PG
TotalSeq™-B0145 anti-human CD103 (Integrin αE)	Ber-ACT8	PG
APC/Fire™ 750 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE/Fire™ 700 anti-human CD103 (Integrin αE) Antibody	Ber-ACT8	FC
TotalSeq™-D0145 anti-human CD103 (Integrin αE)	Ber-ACT8	PG
PE/Fire™ 640 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE anti-human CD103	Ber-ACT8	FC
FITC anti-human CD103	Ber-ACT8	FC
APC anti-human CD103	Ber-ACT8	FC
Spark YG™ 581 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Spark NIR™ 685 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Spark Blue™ 550 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE/Fire™ 810 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
GMP FITC anti-human CD103 (Integrin αE)	Ber-ACT8	FC
GMP PE anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Spark Red™ 718 anti-human CD103 (Integrin αE) (Flexi-Fluor™)	Ber-ACT8	FC
Spark Blue™ 574 anti-human CD103 (Integrin αE) (Flexi-Fluor™)	Ber-ACT8	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12/13/2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

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