GMP PerCP/Cyanine5.5 anti-human CD11b Antibody, CD11b, ICRF44

Clone: ICRF44 (See other available formats)
Other Names: Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

ICRF44_GMP_PerCPCyanine5.5_CD11b_Antibody_051322 — Typical results from human peripheral blood granulocytes stained either with ICRF44 PerCP/Cyanine5.5 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

Compare all formats See GMP PerCP/Cyanine5.5 spectral data

Cat #	Size	Price	Save
260126	100 tests	¥116,160

Description

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as α_M integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β₂ (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.

Product Details

Technical data sheet

Product Details

Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

200 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy⁵, stimulation of monocytes³, blocking of heterotypic PMN aggregation⁸, and blocking of granulocyte activation¹². This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

Application References (PubMed link indicates BioLegend citation)

Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
Rezzonico R, et al. 2001. Blood 97:2932. (Stim)
Marsik C, et al. 2003. Shock 20:493. (FC)
David A, et al. 2003. J. Leukoc. Biol. 74:551. (IF)
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
Thurlow LR, et al. 2010. Infect. Immun. 128:1128. (FC) PubMed
Jadhav S, et al. 2001. J. Immunol. 167:5986. (Block)
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. (FC)
Wen T, et al. 2014. J Immunol. 192:5481. (FC) PubMed
Sprong T, et al. 2003. Blood 102:3702. (Block)
Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
Larsson K, et al. 2015. PNAS. PubMed

Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

Batch-to-batch consistency
Material traceability
Documented procedures
Documented employee training
Equipment maintenance and monitoring records
Lot-specific certificates of analysis
Quality audits per ISO 13485:2016
QA review of released products

Antigen Details

Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β₂ (CD18), 165-170 kD
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells
Function: Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor: ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type: B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690.
Gene ID: 3684 View all products for this Gene ID
UniProt: View information about CD11b on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD11b Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD11b	ICRF44	FC
Biotin anti-human CD11b	ICRF44	FC
PE anti-human CD11b	ICRF44	FC
PE/Cyanine5 anti-human CD11b	ICRF44	FC
Purified anti-human CD11b	ICRF44	FC,CyTOF®,ICC,IHC
Pacific Blue™ anti-human CD11b	ICRF44	FC
Alexa Fluor® 488 anti-human CD11b	ICRF44	FC
Alexa Fluor® 647 anti-human CD11b	ICRF44	FC
PE/Cyanine7 anti-human CD11b	ICRF44	FC
PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
Brilliant Violet 421™ anti-human CD11b	ICRF44	FC,ICC
Brilliant Violet 570™ anti-human CD11b	ICRF44	FC
FITC anti-human CD11b	ICRF44	FC
Brilliant Violet 605™ anti-human CD11b	ICRF44	FC
Brilliant Violet 510™ anti-human CD11b	ICRF44	FC,ICC
Brilliant Violet 650™ anti-human CD11b	ICRF44	FC
Purified anti-human CD11b (Maxpar® Ready)	ICRF44	FC,CyTOF®
Alexa Fluor® 594 anti-human CD11b	ICRF44	ICC,FC
APC/Cyanine7 anti-human CD11b	ICRF44	FC
Brilliant Violet 711™ anti-human CD11b	ICRF44	FC
Brilliant Violet 785™ anti-human CD11b	ICRF44	FC
PE/Dazzle™ 594 anti-human CD11b	ICRF44	FC
APC/Fire™ 750 anti-human CD11b	ICRF44	FC
APC anti-human CD11b	ICRF44	FC
PE anti-human CD11b	ICRF44	FC
TotalSeq™-A0161 anti-human CD11b	ICRF44	PG
Alexa Fluor® 700 anti-human CD11b	ICRF44	FC
PE/Cyanine7 anti-human CD11b	ICRF44	FC
TotalSeq™-B0161 anti-human CD11b	ICRF44	PG
TotalSeq™-C0161 anti-human CD11b	ICRF44	PG
PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
Ultra-LEAF™ Purified anti-human CD11b	ICRF44	FC,CyTOF®,Block,ICC,IHC
TotalSeq™-D0161 anti-human CD11b	ICRF44	PG
GMP PE/Cyanine7 anti-human CD11b	ICRF44	FC
Pacific Blue™ anti-human CD11b	ICRF44	FC
GMP PE anti-human CD11b	ICRF44	FC
Spark UV™ 387 anti-human CD11b	ICRF44	FC
GMP PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
FITC anti-human CD11b	ICRF44	FC
APC/Fire™ 750 anti-human CD11b	ICRF44	FC
GMP APC anti-human CD11b	ICRF44	FC
GMP FITC anti-human CD11b	ICRF44	FC
GMP APC/Fire™ 750 anti-human CD11b	ICRF44	FC
Spark Blue™ 515 anti-human CD11b	ICRF44	FC
Spark Violet™ 500 anti-human CD11b	ICRF44	FC
Spark Red™ 718 anti-human CD11b (Flexi-Fluor™)	ICRF44	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/08/2022

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.