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Intracellular Staining Permeabilization Wash Buffer (10X)

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Other Names
Perm/Wash, Permeabilization Buffer
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421002 100 mL ¥20,680
Description

Intracellular Staining Permeabilization Wash Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Intracellular Staining Permeabilization Wash Buffer is used to permeabilize cells following fixation with Intracellular Staining Fixation Buffer (Cat. No. 420801). It is supplied as a 10X solution and should be diluted in deionized water prior to use. Intracellular Staining Permeabilization Wash Buffer has been formulated to have minimal effects on cells, reduce non-specific staining and enhance the signal to noise ratio. It can be used for antibody dilutions and cell washing during intracellular staining.

Product Details
Technical data sheet

Product Details

Storage & Handling
The Intracellular Staining Perm wash buffer solution should be stored between 2°C and 8°C. Do not freeze.
Application

ICC, ICFC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

For use in permeabilization, dilute Intracellular Staining Permeabilization Wash Buffer (10X) to 1X in DI water. Resuspend fixed cells in diluted Intracellular Staining Permeabilization Wash Buffer and centrifuge at 350 xg for 5-10 minutes, and repeat the process twice. It is recommended that the reagent be titrated for optimal performance for each application. Please see "Intracellular Cytokine Staining Protocol" on BioLegend's website for more details.

Application Notes

The Intracellular Staining Permeabilization Wash Buffer (10X) may have crystallization or precipitation observed when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is heavy precipitation observed after dilution to 1X working solution, the buffer can be filtered to clarify the solution.

Application References

(PubMed link indicates BioLegend citation)
  1. Smeltz RB. 2007. J. Immunol. 178:4786.
  2. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  3. del Rio ML, et al. 2011. Transplantation. 92:1085. PubMed
  4. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  5. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  6. Xiao Z, et al. 2013. Mol Immunol. 56:423. PubMed
  7. Kusner LL, et al. 2014. PLoS One. 9:102231. PubMed
  8. Ni PP, et al. 2014. J Immunol. 193:1778. PubMed
Product Citations
  1. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  2. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  3. Kim S, et al. 2020. Development. 147:00:00. PubMed
  4. Dumauthioz N, et al. 2020. Cell Mol Immunol. . PubMed
  5. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  6. Hanga MP, et al. 2021. Biotechnol Bioeng. 118:3175. PubMed
  7. Arbues A, et al. 2020. PLoS Pathog. 16:e1008312. PubMed
  8. Kim J, et al. 2021. Cytometry A. 99:807. PubMed
  9. Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed
  10. Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed
  11. Nozaki K, et al. 2022. Nature. 606:960. PubMed
  12. Shemesh A, et al. 2022. J Exp Med. 219: . PubMed
  13. Yin XY, et al. 2023. Neural Regen Res. 18:213. PubMed
  14. Blanas A, et al. 2022. iScience. 25:104719. PubMed
  15. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  16. Gong YY, et al. 2022. Cancer Res Commun. 2:842. PubMed
  17. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  18. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  19. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  20. Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed
  21. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  22. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  23. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  24. Chan L, et al. 2022. Cells. 12: . PubMed
  25. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  26. Qin S, et al. 2023. Acta Pharm Sin B. :. PubMed
  27. Sun L, et al. 2023. Mol Med Rep. 27: . PubMed
  28. Di Benedetto C, et al. 2023. Cancers (Basel). 15: . PubMed
  29. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  30. Xiao JF, et al. 2022. Mol Cancer Res. 20:637. PubMed
  31. Shen JZ, et al. 2022. Mol Cell. 82:1123. PubMed
  32. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  33. Zhang H, et al. 2022. J Exp Med. 219:. PubMed
  34. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  35. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  36. Chen R, et al. 2022. J Mol Cell Biol. 14:. PubMed
  37. Tang J, et al. 2022. Sci Immunol. 7:eadd4853. PubMed
  38. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  39. Hajnik RL, et al. 2022. Sci Transl Med. 14:eabq1945. PubMed
  40. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  41. Li L, et al. 2022. Cancer Immunol Res. 10:1475. PubMed
  42. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  43. Stokes JV, et al. 2022. Curr Protoc. 2:e584. PubMed
  44. Jia DJ, et al. 2022. Gut Microbes. 14:2145843. PubMed
  45. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  46. Kodama N, et al. 2023. Front Nutr. 10:1081263. PubMed
  47. Mohamed GA, et al. 2023. Breast Cancer Res. 25:23. PubMed
  48. Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed
  49. Wang YH, et al. 2023. Int J Mol Sci. 24:. PubMed
  50. Deng G, et al. 2023. Cancers (Basel). 15:. PubMed
  51. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  52. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
  53. Ruan H, et al. 2023. Acta Pharm Sin B. 13:2202. PubMed
  54. Zhang TR, et al. 2023. Pharmaceuticals (Basel). 16:. PubMed
  55. Komoto S, et al. 2023. Cancers (Basel). 15:. PubMed
  56. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  57. van Vloten JP, et al. 2022. J Immunother Cancer. 10:. PubMed
  58. Lebedeva IV, et al. 2021. Cell Death Dis. 12:770. PubMed
  59. Zhang L, et al. 2021. J Immunol. 207:590. PubMed
  60. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  61. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  62. Glaser K, et al. 2015. Immunol Lett. 166: 19-27. PubMed
  63. Kusner L, et al. 2014. PLoS One. 9:102231. PubMed
  64. Khayrullina G, et al. 2020. Skelet Muscle. 10:16. PubMed
  65. Spitzer SO, et al. 2019. Neuron. 101:459. PubMed
  66. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  67. Huang X, et al. 2019. Cancer Immunol Res. 1.388888889. PubMed
  68. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  69. Jacob A, et al. 2020. Nature Protocols. 14(12):3303-3332.. PubMed
  70. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  71. Stewart CA, et al. 2020. bioRxiv. . PubMed
  72. Stewart CA, et al. 2021. J Thorac Oncol. Online ahead of prin. PubMed
  73. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  74. Poli V, et al. 2021. iScience. 24:103256. PubMed
  75. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  76. Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
  77. Zhang S, et al. 2022. Cell Death Dis. 13:844. PubMed
  78. Tsai CY, et al. 2022. J Biomed Sci. 29:47. PubMed
  79. Gärtner F, et al. 2021. Front Immunol. 12:745132. PubMed
  80. Gao F, et al. 2021. Front Cell Infect Microbiol. 10:593805. PubMed
  81. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  82. Martinez-Usatorre A, et al. 2020. Front Immunol. 11:340. PubMed
  83. Martinez–Usatorre A, et al. 2019. Cancer Immunol Res. 0.995138889. PubMed
  84. Goyal S, et al. 2017. Sci Rep. . 10.1038/s41598-017-18046-x. PubMed
  85. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  86. Shinde MY, et al. 2017. J Biol Chem. 292:18240. PubMed
  87. Haryadi L, et al. 2019. Vet World. 12:1529. PubMed
  88. Ghose J, et al. 2018. Oncoimmunology. 7:e1486948. PubMed
  89. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  90. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  91. Herda S, et al. 2021. Int J Cancer. 148:3097. PubMed
  92. Lau H, et al. 2021. Int J Mol Sci. 22:. PubMed
  93. Zhou H, et al. 2022. J Clin Invest. 132:. PubMed
  94. Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed
  95. Lau H, et al. 2021. Xenotransplantation. 28:e12667. PubMed
  96. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  97. Ma Q, et al. 2022. Nat Commun. 13:4142. PubMed
  98. Datta A, et al. 2022. J Fungi (Basel). 8:. PubMed
  99. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  100. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  101. Hendricks A, et al. 2021. Front Oncol. 11:646885. PubMed
  102. Shamsi F, et al. 2021. Nat Metab. 3:485. PubMed
  103. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  104. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  105. Sohrabi Y, et al. 2020. Front Immunol. 11:353. PubMed
  106. Martinez–Usatorre A, et al. 2018. J Immunol. 201:792. PubMed
  107. Clancy–Thompson E, et al. 2018. Cancer Immunol Res. 1.308333333. PubMed
  108. Xiao Z, et al. 2013. Mol Immunol. 56:423. PubMed
  109. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  110. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  111. Zhang X, et al. 2022. Theranostics. 12:1621. PubMed
  112. Berical A, et al. 2022. Nat Commun. 13:4270. PubMed
  113. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  114. Rodriguez S, et al. 2020. Xenotransplantation. 27:e12554. PubMed
  115. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  116. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  117. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  118. Pearce V, et al. 2015. J Immunol. 195: 3206 - 3217. PubMed
  119. Baek J, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.02.011. PubMed
  120. Lownik JC, et al. 2019. J Immunol. 202:664. PubMed
  121. Lau H, et al. 2020. Xenotransplantation. 27:e12555. PubMed
  122. Mkhikian H, et al. 2022. Nat Aging. 2:231. PubMed
  123. Li J, et al. 2022. J Tissue Eng. 13:20417314221127908. PubMed
  124. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  125. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  126. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  127. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  128. Alvandi Z, et al. 2022. J Am Heart Assoc. 11:e023695. PubMed
  129. van Duijn A, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  130. Jakic B, et al. 2021. Cell Death Dis. 12:187. PubMed
  131. Ouyang W, et al. 2021. Invest Ophthalmol Vis Sci. 62:25:00. PubMed
  132. Nausch N, et al. 2017. PLoS Negl Trop Dis. 11(2):e0005415. PubMed
  133. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  134. Breznik JA, et al. 2018. Physiol Rep. 6:e13937. PubMed
  135. Li Q, et al. 2017. Front Immunol. 0.797916667. PubMed
  136. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  137. Gunasena M, et al. 2022. Sci Rep. 12:12377. PubMed
  138. Hayes D, et al. 2022. JCI Insight. 7:. PubMed
  139. Gong C, et al. 2021. Mol Cell. 81:4059. PubMed
  140. Chen DL, et al. 2021. Mol Cancer. 20:166. PubMed
  141. Kim S, et al. 2020. Development. . PubMed
  142. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  143. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  144. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  145. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  146. Bajpai G, et al. 2018. Nat Med. 24:1234. PubMed
  147. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  148. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  149. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  150. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  151. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  152. Wen T, et al. 2022. Cancer Immunol Res. 10:162. PubMed
  153. Ma K, et al. 2022. iScience. 25:104347. PubMed
  154. Dong M, et al. 2022. J Immunother Cancer. 10:. PubMed
  155. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  156. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  157. Haswell JR, et al. 2021. PLoS One. 16:e0252848. PubMed
  158. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  159. Lau H, et al. 2020. Islets. 41:12. PubMed
  160. Lau H, et al. 2020. PLoS One. 15:e0243506. PubMed
  161. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  162. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  163. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  164. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  165. Park SR, et al. 2020. Cell Rep. 32:108077. PubMed
  166. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  167. Spiliopoulou P, et al. 2022. Mol Cancer Ther. . PubMed
  168. Tintos-Hernandez JA, et al. 2021. Brain Commun. 3:fcab215. PubMed
  169. Koutník J, et al. 2022. Cell Commun Signal. 20:54. PubMed
  170. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  171. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  172. Gao J, et al. 2022. Front Immunol. 13:955812. PubMed
  173. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  174. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  175. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  176. Huang T, et al. 2022. Nat Commun. 13:3489. PubMed
  177. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  178. DiMuzio JM, et al. 2021. Vaccine X. 8:100098. PubMed
  179. Lee G, et al. 2021. Viruses. 13: . PubMed
  180. Lei L, et al. 2021. Front Cell Dev Biol. 659744:9. PubMed
  181. Frafjord A, et al. 2020. Scand J Immunol. e12889:92. PubMed
  182. Lownik JC, et al. 2020. Biochem Biophys Rep. 24:100803. PubMed
  183. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  184. Sun X, et al. 2017. Microbes Infect. 10.1016/j.micinf.2017.08.007. PubMed
  185. Hernandez AA, et al. 2020. J Immunol. 205:2806. PubMed
  186. Su Y, et al. 2020. Cell. 1479:183. PubMed
  187. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  188. Mcgaw C, et al. 2022. Nat Commun. 13:2833. PubMed
  189. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  190. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  191. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  192. Wang X, et al. 2022. Elife. 11:. PubMed
  193. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  194. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  195. Zheng L, et al. 2020. Clin Cancer Res. 26:3694. PubMed
  196. Witt G, et al. 2020. Cell Biol Toxicol. . PubMed
  197. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  198. Giadone RM, et al. 2018. Amyloid. 25:148. PubMed
  199. Gebremeskel S, et al. 2017. Cancer Immunol Res. 0.9625. PubMed
  200. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  201. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  202. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  203. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  204. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  205. Luvanda MK, et al. 2021. J Fungi (Basel). 7:. PubMed
  206. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  207. Assadi G, et al. 2016. PLoS One. 11:e0168276. PubMed
  208. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  209. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA
Go To Top Version: 3    Revision Date: 08/26/2015

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