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- Clone
- CARL-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TNF-like weak inducer of apoptosis, Apo-3 ligand (Apo3L), Death receptor 3-L (DR3-L), TNFSF 12, CD255
- Isotype
- Mouse IgG3, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human TWEAK transfected cells stained with Carl-1 PE
TNF-like weak inducer of apoptosis (TWEAK) is a type II transmembrane TNF superfamily member with strong homology to TNF in its extracellular portion, though less potent in inducing apoptosis. It has been clustered as CD255. TWEAK transcript is expressed broadly in many adult and fetal tissues; however, the staining of human peripheral blood mononuclear cells with TWEAK-specific antibodies shows a more restricted pattern. TWEAK is expressed as membrane-bound and secreted forms. Interaction of TWEAK with its receptors (DR3, Fn14) promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, and apoptosis in a number of human cell lines.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- hTWEAK/2PK-3 transfectant cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 308304) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
ELISA Detection: The biotinylated CARL-1 antibody is useful as the detection antibody in sandwich ELISA for measuring human TWEAK protein, in combination with CARL-2 antibody (Cat. No. 308402) used as ELISA capture.
Neutralization1,2: In a dose-dependent manner, CARL-1 is more effective than CARL-21. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 308307). -
Application References
(PubMed link indicates BioLegend citation) -
- Nakayama, M., et al. 2000. J. Exp. Med. 192:1373. (Neut)
- Nakayama, M., et al. 2003. J. Immunol. 170:341. (Neut)
- Zou H, et al. 2011. Gynecol Oncol.123:123. PubMed.
- Product Citations
-
- RRID
-
AB_314709 (BioLegend Cat. No. 308305)
Antigen Details
- Structure
- TNF superfamily; 17 kD (Mammalian)
- Distribution
-
Normal tissue and tumor cells at low levels
- Function
- Promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, apoptosis
- Bioactivity
- Weak apoptotic induction; mediate CD4+ T-cell killing of macrophages; induces IL-6, IL-8, ICAM-1 in astrocytes; HUVEC proliferation
- Cell Sources
- Transcripts widely expressed and abundant in most tissues; protein efficiently secreted
- Cell Targets
- Macrophages, astrocytes, endothelial cells
- Receptors
- Apo-3 (DR3, WSL-1, TRAMP, LARD); TWEAKR
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Chicheportiche, Y., et al. 1997. J. Biol. Chem. 272:32401.
3. Kaplan, M., et al. 2000. J. Immunol. 164:2897.
4. Saas, P., et al. 2000. Glia 32:102.
5. Wiley, S., et al. 2001. Immunity 15:837. - Regulation
- Type II integral membrane protein readily cleaved from cell surface
- Gene ID
- 8742 View all products for this Gene ID
- UniProt
- View information about CD255 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD255 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD255 (TWEAK) | CARL-1 | FC |
| Purified anti-human CD255 (TWEAK) | CARL-1 | FC,IHC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD255 (TWEAK)
Human TWEAK transfected cells stained with Carl-1 PE -
Purified anti-human CD255 (TWEAK)
Human TWEAK transfected cells stained with Carl-1 PE
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