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Purified anti-human CD255 (TWEAK) Antibody

Pricing & Availability
Clone
CARL-1 (See other available formats)
Regulatory Status
RUO
Other Names
TNF-like weak inducer of apoptosis, Apo-3 ligand (Apo3L), Death receptor 3-L (DR3-L), TNFSF 12, CD255
Isotype
Mouse IgG3, κ
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Product Citations
publications
Carl-1
Human TWEAK transfected cells stained with Carl-1 PE
  • Carl-1
    Human TWEAK transfected cells stained with Carl-1 PE
Cat # Size Price Save
308302 100 µg ¥42,680
Description

TNF-like weak inducer of apoptosis (TWEAK) is a type II transmembrane TNF superfamily member with strong homology to TNF in its extracellular portion, though less potent in inducing apoptosis. It has been clustered as CD255. TWEAK transcript is expressed broadly in many adult and fetal tissues; however, the staining of human peripheral blood mononuclear cells with TWEAK-specific antibodies shows a more restricted pattern. TWEAK is expressed as membrane-bound and secreted forms. Interaction of TWEAK with its receptors (DR3, Fn14) promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, and apoptosis in a number of human cell lines.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
hTWEAK/2PK-3 transfectant cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by sandwich ELISA. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 308304) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
ELISA Detection: The biotinylated CARL-1 antibody is useful as the detection antibody in sandwich ELISA for measuring human TWEAK protein, in combination with CARL-2 antibody (Cat. No. 308402) used as ELISA capture.
Neutralization1,2: In a dose-dependent manner, CARL-1 is more effective than CARL-21. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 308307).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakayama, M., et al. 2000. J. Exp. Med. 192:1373. (Neut)
  2. Nakayama, M., et al. 2003. J. Immunol. 170:341. (Neut)
  3. Zou H, et al. 2011. Gynecol Oncol.123:123. PubMed.
Product Citations
  1. Loi M, et al. 2019. Methods Mol Biol. 455:1880. PubMed
  2. Zou H, et al. 2011. Gynecol Oncol. 123:123. PubMed
RRID
AB_314706 (BioLegend Cat. No. 308302)

Antigen Details

Structure
TNF superfamily; 17 kD (Mammalian)
Distribution

Normal tissue and tumor cells at low levels

Function
Promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, apoptosis
Bioactivity
Weak apoptotic induction; mediate CD4+ T-cell killing of macrophages; induces IL-6, IL-8, ICAM-1 in astrocytes; HUVEC proliferation
Cell Sources
Transcripts widely expressed and abundant in most tissues; protein efficiently secreted
Cell Targets
Macrophages, astrocytes, endothelial cells
Receptors
Apo-3 (DR3, WSL-1, TRAMP, LARD); TWEAKR
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Chicheportiche, Y., et al. 1997. J. Biol. Chem. 272:32401.
3. Kaplan, M., et al. 2000. J. Immunol. 164:2897.
4. Saas, P., et al. 2000. Glia 32:102.
5. Wiley, S., et al. 2001. Immunity 15:837.

Regulation
Type II integral membrane protein readily cleaved from cell surface
Gene ID
8742 View all products for this Gene ID
UniProt
View information about CD255 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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