- Clone
- 3D11/HLA-F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HLAF, CDA12, HLA-5.4, HLA-CDA12
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![3D11slashHLA-F_PE_HLA-F_Antibody_1_022417 3D11slashHLA-F_PE_HLA-F_Antibody_1_022417](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/3D11slashHLA-F_PE_HLA-F_Antibody_1_022417.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes surface stained with CD20 Pacific Blue™ and HLA-F (clone3D11/HLA-F) PE (top) or mouse IgG1, κ PE isotype control (bottom). -
Cat # | Size | Price | Save |
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373203 | 25 tests | ¥31,020 | |
373204 | 100 tests | ¥73,700 |
A 42kD member of non-classical or class Ib human MHC family. It exhibits low allelic polymorphism and shorter cytoplasmic tail due to absence of exon seven. It is expressed irrespective of bound peptide. It is primarily found intracellularly on Endoplasmic Reticulum and Golgi membrane and its surface expression increases in correlation with classical MHC-I up regulation in activated monocytes, B, T, and NK cells. It is involved in inflammatory responses. It stabilizes the unstable MHC-I Open Conformers (OC) and facilitates uptake of extracellular antigen for cross presentation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Complex mixed with IFA, complex without adjuvant.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Ishitani A, et al. 2003. J. Immunol. 171:1376.
- Product Citations
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- RRID
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AB_2650871 (BioLegend Cat. No. 373203)
AB_2650872 (BioLegend Cat. No. 373204)
Antigen Details
- Structure
- 42 kD Single pass type I membrane protein, heterodimer of an alpha and a Beta chain.
- Distribution
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B, NK, monocyte and T cells.
- Function
- MHC-I OC stabilization, antigen cross presentation.
- Ligand/Receptor
- KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, TAP.
- Cell Type
- B cells, Monocytes, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- MHC Antigens
- Antigen References
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- Goodridge JP, et al. 2013. J. Immunol. 191:1567.
- Lepin EJ, et al. 2000. Eur. J. Immunol. 30:3552.
- Burian A,et al. 2016. PLoS One 11:e0163297.
- Lee N, et al. 2010. Eur. J. Immunol. 40:2308.
- Gene ID
- 3134 View all products for this Gene ID
- UniProt
- View information about HLA-F on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All HLA-F Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human HLA-F | 3D11/HLA-F | FC,Direct ELISA,IHC-F,IP |
PE anti-human HLA-F | 3D11/HLA-F | FC |
PE/Dazzle™ 594 anti-human HLA-F | 3D11/HLA-F | FC |
APC anti-human HLA-F | 3D11/HLA-F | FC |
TotalSeq™-C1047 anti-human HLA-F | 3D11/HLA-F | PG |
TotalSeq™-B1047 anti-human HLA-F | 3D11/HLA-F | PG |
TotalSeq™-D1047 anti-human HLA-F | 3D11/HLA-F | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human HLA-F
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
PE anti-human HLA-F
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
PE/Dazzle™ 594 anti-human HLA-F
PMA+Ionomycin-stimulated (6 hours) human peripheral blood ly... -
APC anti-human HLA-F
PMA+Ionomycin-stimulated (6 hours) human peripheral blood ly... -
TotalSeq™-C1047 anti-human HLA-F
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TotalSeq™-B1047 anti-human HLA-F
-
TotalSeq™-D1047 anti-human HLA-F
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