PE/Dazzle 594 anti-human HLA-F Antibody anti-HLA-F

Clone: 3D11/HLA-F (See other available formats)
Regulatory Status: RUO
Other Names: HLAF, CDA12, HLA-5.4, HLA-CDA12
Isotype: Mouse IgG1, κ
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3D11slashHLA-F_PEDazzle594_HLA-F_Antibody_060718 — PMA+Ionomycin-stimulated (6 hours) human peripheral blood lymphocytes was stained with CD20 FITC and HLA-F (clone 3D11/HLA-F, left) PE/Dazzle™ 594 or Mouse IgG1, κ isotype control (right) PE/Dazzle™ 594.

Compare all formats See PE/Dazzle™ 594 spectral data

Cat #	Size	Price	Save
373205	25 tests	¥37,620
373206	100 tests	¥84,040

Description

A 42kD member of non-classical or class Ib human MHC family. It exhibits low allelic polymorphism and shorter cytoplasmic tail due to absence of exon seven. It is expressed irrespective of bound peptide. It is primarily found intracellularly on Endoplasmic Reticulum and Golgi membrane and its surface expression increases in correlation with classical MHC-I up regulation in activated monocytes, B, T, and NK cells. It is involved in inflammatory responses. It stabilizes the unstable MHC-I Open Conformers (OC) and facilitates uptake of extracellular antigen for cross presentation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Complex mixed with IFA, complex without adjuvant.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2734427 (BioLegend Cat. No. 373205)
AB_2734427 (BioLegend Cat. No. 373206)

Antigen Details

Structure: 42 kD Single pass type I membrane protein, heterodimer of an alpha and a Beta chain.
Distribution: B, NK, monocyte and T cells.
Function: MHC-I OC stabilization, antigen cross presentation.
Ligand/Receptor: KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, TAP.
Cell Type: B cells, Monocytes, NK cells, T cells
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: Goodridge JP, et al. 2013. J. Immunol. 191:1567.

Lepin EJ, et al. 2000. Eur. J. Immunol. 30:3552.

Burian A,et al. 2016. PLoS One 11:e0163297.

Lee N, et al. 2010. Eur. J. Immunol. 40:2308.
Gene ID: 3134 View all products for this Gene ID
UniProt: View information about HLA-F on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All HLA-F Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human HLA-F	3D11/HLA-F	FC,Direct ELISA,IHC-F,IP
PE anti-human HLA-F	3D11/HLA-F	FC
PE/Dazzle™ 594 anti-human HLA-F	3D11/HLA-F	FC
APC anti-human HLA-F	3D11/HLA-F	FC
TotalSeq™-C1047 anti-human HLA-F	3D11/HLA-F	PG
TotalSeq™-B1047 anti-human HLA-F	3D11/HLA-F	PG
TotalSeq™-D1047 anti-human HLA-F	3D11/HLA-F	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/07/2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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