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PE anti-mouse CD48 Antibody

Pricing & Availability
Clone
HM48-1 (See other available formats)
Regulatory Status
RUO
Other Names
BCM1, SLAMF2, Blast-1
Isotype
Armenian Hamster IgG
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Product Citations
publications
HM48-1_PE_090507
C57BL/6 mouse splenocytes stained with HM48-1 PE
  • HM48-1_PE_090507
    C57BL/6 mouse splenocytes stained with HM48-1 PE
Compare all formats See PE spectral data
Cat # Size Price Save
103405 50 µg ¥21,720
103406 200 µg ¥62,840
Description

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T lymphoma MBL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
  2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
Product Citations
  1. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  2. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  3. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  4. Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed
  5. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  6. Wu N, et al. 2020. Cell Rep. 30:1129. PubMed
  7. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  8. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  9. Matsushita Y, et al. 2021. J Bone Miner Res. 36:1145. PubMed
  10. Wong J, et al. 2015. Elife. 3: 07839. PubMed
  11. McCabe A, et al. 2020. STAR Protocols. 33(7):2294-305. PubMed
  12. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  13. Liu J, et al. 2012. PLoS One. 7:e44044. PubMed
  14. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  15. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  16. Ise W, et al. 2018. Immunity. 48:702. PubMed
  17. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  18. Wu G, et al. 2022. Wellcome Open Res. 6:134. PubMed
  19. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  20. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  21. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  22. Xie X, et al. 2015. Sci Rep. 5: 18115. PubMed
  23. Ueda K, et al. 2021. Cancer Cell. 39(4):529-547.e7. PubMed
  24. Revollo J, et al. 2015. Mutagenesis. 30:315. PubMed
  25. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  26. Zhang B, et al. 2021. Nature. 599:471. PubMed
  27. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
RRID
AB_313020 (BioLegend Cat. No. 103405)
AB_313020 (BioLegend Cat. No. 103406)

Antigen Details

Structure
Ig superfamily, 45 kD
Distribution

T cells and B cells, monocytes/macrophages

Function
Adhesion, T cell recognition
Ligand/Receptor
CD2, CD244
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
12506 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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