PE/Fire™ 640 anti-human CD154 Antibody, CD154, 24-31

Clone: 24-31 (See other available formats)
Regulatory Status: RUO
Other Names: CD40L, gp39, TRAP, T-BAM, TNFSF5
Isotype: Mouse IgG1, κ
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Product Citations: publications

24_31_PEFire640_CD154_Antibody_111623 — Human peripheral blood lymphocytes were stimulated with PMA+ionomycin (6 hrs.) and stained with anti-human CD3 (clone UCHT1) APC and anti-human CD154 (clone 24-31) PE/Fire™ 640 (left) or mouse IgG1, κ PE/Fire™ 640 isotype control (right).

Compare all formats See PE/Fire™ 640 spectral data

Cat #	Size	Price	Save
310855	25 tests	¥37,620
310856	100 tests	¥84,040

Description

CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy^1,3 and blocking of T cell-dependent B cell differentiation^1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
RRID: AB_3083301 (BioLegend Cat. No. 310855)
AB_3083301 (BioLegend Cat. No. 310856)

Antigen Details

Structure: TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
Distribution: Activated T cells
Function: B cell costimulation
Ligand/Receptor: CD40
Cell Type: T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227.
2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275.
3. Ford G, et al. 1999. J. Immunol. 162:4037.
Gene ID: 959 View all products for this Gene ID
UniProt: View information about CD154 on UniProt.org

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

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Other Formats

View All CD154 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD154	24-31	FC,IHC-F
FITC anti-human CD154	24-31	FC,IHC-F
PE anti-human CD154	24-31	FC
PE/Cyanine5 anti-human CD154	24-31	FC
APC anti-human CD154	24-31	FC
Biotin anti-human CD154	24-31	FC,IHC-F
Alexa Fluor® 488 anti-human CD154	24-31	FC,IHC-F
Alexa Fluor® 647 anti-human CD154	24-31	FC,IHC-F
Pacific Blue™ anti-human CD154	24-31	FC
Brilliant Violet 785™ anti-human CD154	24-31	FC
APC/Cyanine7 anti-human CD154	24-31	FC
Brilliant Violet 421™ anti-human CD154	24-31	FC,IHC-F
Brilliant Violet 605™ anti-human CD154	24-31	FC
Ultra-LEAF™ Purified anti-human CD154	24-31	FC,Block,IHC-F
Brilliant Violet 510™ anti-human CD154	24-31	FC,IHC-F
PE/Cyanine7 anti-human CD154	24-31	FC
PerCP/Cyanine5.5 anti-human CD154	24-31	FC
Brilliant Violet 711™ anti-human CD154	24-31	FC
Purified anti-human CD154 (Maxpar® Ready)	24-31	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD154	24-31	FC
TotalSeq™-A0032 anti-human CD154	24-31	PG
Alexa Fluor® 700 anti-human CD154	24-31	FC
APC/Fire™ 750 anti-human CD154	24-31	FC
TotalSeq™-C0032 anti-human CD154	24-31	PG
TotalSeq™-B0032 anti-human CD154	24-31	PG
TotalSeq™-D0032 anti-human CD154	24-31	PG
PE/Fire™ 640 anti-human CD154	24-31	FC
PE/Fire™ 810 anti-human CD154	24-31	FC
PerCP/Fire™ 780 anti-human CD154	24-31	FC
PE/Fire™ 700 anti-human CD154	24-31	FC
Spark Red™ 718 anti-human CD154 (Flexi-Fluor™)	24-31	FC
Brilliant Violet 650™ anti-human CD154	24-31	FC
Spark Blue™ 574 anti-human CD154 (Flexi-Fluor™)	24-31	FC
Spark Blue™ 550 anti-human CD154 (Flexi-Fluor™)	24-31	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07/21/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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