PerCP anti-human CD19 Antibody anti-CD19

Clone: 4G7 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: B4, CVID3
Isotype: Mouse IgG1, κ
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Product Citations: publications

4G7_PerCP_CD19_Antibody_073118 — Human peripheral blood lymphocytes were stained with CD19 (clone 4G7, filled histogram) PerCP or mouse IgG1, κ isotype control (open histogram) PerCP.

Compare all formats See PerCP spectral data

Cat #	Size	Price	Save
392509	25 tests	¥34,980
392510	100 tests	¥76,340

Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Chronic Lymphocytic Leukemia (CLL) cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone 4G7 partially blocks anti-human CD19 clones HIB19 and SJ25C1 staining based on in-house testing
RRID: AB_2750100 (BioLegend Cat. No. 392509)
AB_2750100 (BioLegend Cat. No. 392510)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution: Normal and malignant B lymphocytes
Function: B cell activation and differentiation
Ligand/Receptor: Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type: B cells, Lymphocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Tedder T, et al. 1994. Immunol. Today 15:437.

Bradbury L, et al. 1993. J. Immunol. 151:2915.
Gene ID: 930 View all products for this Gene ID
UniProt: View information about CD19 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD19 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD19	4G7	FC
APC anti-human CD19	4G7	FC
PE anti-human CD19	4G7	FC
FITC anti-human CD19	4G7	FC
PerCP anti-human CD19	4G7	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 07/31/2018

For Research Use Only. Not for diagnostic or therapeutic use.

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