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- Clone
- 4G7 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- B4, CVID3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were stained with purified CD19 (clone 4G7) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG-FITC.
CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Chronic Lymphocytic Leukemia (CLL) cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone 4G7 partially blocks anti-human CD19 clones HIB19 and SJ25C1 staining based on in-house testing
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Application References
(PubMed link indicates BioLegend citation) -
- Cabezudo E, et al. 1999. Haematologica. 84:413-8
- Van Zelm MC et al. 2010. J Clin Invest. 120:1265-74
- Product Citations
-
- RRID
-
AB_2721738 (BioLegend Cat. No. 392502)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 95 kD
- Distribution
-
Normal and malignant B lymphocytes
- Function
- B cell activation and differentiation
- Ligand/Receptor
- Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
- Cell Type
- B cells, Lymphocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Tedder T, et al. 1994. Immunol. Today 15:437.
- Bradbury L, et al. 1993. J. Immunol. 151:2915.
- Gene ID
- 930 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
Related FAQs
Other Formats
View All CD19 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD19 | 4G7 | FC |
| APC anti-human CD19 | 4G7 | FC |
| PE anti-human CD19 | 4G7 | FC |
| FITC anti-human CD19 | 4G7 | FC |
| PerCP anti-human CD19 | 4G7 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD19
Human peripheral blood lymphocytes were stained with purifie... -
APC anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
PE anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
FITC anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
PerCP anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c...
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