PerCP anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
30-F11_PerCP_011508
C57BL/6 mouse splenocytes stained with 30-F11 PerCP
  • 30-F11_PerCP_011508
    C57BL/6 mouse splenocytes stained with 30-F11 PerCP
Compare all formats See PerCP spectral data
Cat # Size Price Save
103129 25 µg ¥19,050
103130 100 µg ¥51,980
Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Filipello F et al. 2018. Immunity. 48(5):979-991 . PubMed
  2. Haratani K, et al. 2019. J Clin Invest. 130:374. PubMed
  3. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  4. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  5. Huang N, et al. 2022. Stem Cells Int. 2022:9977610. PubMed
  6. Xiong T, et al. 2022. Cancer Sci. 113:1955. PubMed
  7. Sefik E, et al. 2022. Nature. 606:585. PubMed
  8. Lees-Shepard JB, et al. 2022. J Clin Invest. 132: . PubMed
  9. Aghayev T, et al. 2022. Cancer Discov. 12:1960. PubMed
  10. Campisi L, et al. 2022. Nature. 606:945. PubMed
  11. Li Y, et al. 2023. Bioact Mater. 20:548. PubMed
  12. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  13. Pingul BY, et al. 2022. iScience. 25:105139. PubMed
  14. Wei M, et al. 2022. Front Immunol. 13:1017574. PubMed
  15. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  16. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  17. Quispe Calla N, et al. 2016. Sci Rep. 6:37723. PubMed
  18. Gao T, et al. 2022. Cell Biochem Biophys. 80:579. PubMed
  19. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  20. Chung KJ, et al. 2023. Int J Mol Sci. 24:. PubMed
  21. Huang T, et al. 2023. Front Immunol. 14:1124152. PubMed
  22. Ji Y, et al. 2023. Nat Cell Biol. 25:726. PubMed
  23. Bo Yu et al. 2018. Cell stem cell. 23(2):193-209 . PubMed
  24. Keeter WC, et al. 2022. Eur Heart J Open. 2:oeac028. PubMed
  25. Di Martile M, et al. 2020. J Immunother Cancer. 8:. PubMed
  26. Zhou J, et al. 2021. Int J Med Sci. 18:3516. PubMed
  27. Sun J, et al. 2020. Acta Biomater. 289:106. PubMed
  28. Shin J, et al. 2016. Sci Rep. 6:23426. PubMed
  29. Kawashima N, et al. 2022. Nat Commun. 13:1624. PubMed
  30. Lopez-Guadamillas E, et al. 2016. Sci Rep. 6:34542. PubMed
  31. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  32. Stolp B, et al. 2022. Cell Rep. 38:110387. PubMed
  33. Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed
  34. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  35. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  36. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  37. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  38. Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed
  39. Tajfirouz D, et al. 2017. Virology. 10.1016/j.virol.2017.02.022. PubMed
  40. Peng L, et al. 2020. Transl Lung Cancer Res. 0.892361111. PubMed
  41. Liu YJ, et al. 2020. Theranostics. 10:5225. PubMed
  42. Nguyen CM, et al. 2019. Diabetes. 68:1499. PubMed
  43. Zhang C, et al. 2021. Clin Transl Immunology. 10:e1310. PubMed
  44. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  45. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  46. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  47. Law HL, et al. 2020. Front Pharmacol. 11:274. PubMed
  48. Sears SM, et al. 2020. Am J Physiol Renal Physiol. 319:F674. PubMed
  49. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  50. Gabbita S, et al. 2015. PLoS One. 10: e0137305. PubMed
  51. Taube C, et al. 2011. PLoS One. 6:e21799. PubMed
  52. Yang S, et al. 2019. Nat Commun. 10:2782. PubMed
  53. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  54. Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed
  55. Amo-Aparicio J, et al. 2021. Theranostics. 11:9805. PubMed
  56. Saitoh BY, et al. 2021. Brain Behav Immun. 95:362. PubMed
  57. Kumaran Satyanarayanan S, et al. 2019. Nat Commun. 10:3471. PubMed
  58. Zou J, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:983. PubMed
  59. Aebischer D, et al. 2014. PLoS One. 9:99297. PubMed
  60. Everard A, et al. 2014. Nat Commun. 5:5648. PubMed
  61. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  62. Demandt JAF, et al. 2021. Frontiers in Cell and Developmental Biology. 9:664258. PubMed
  63. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  64. Wang F, et al. 2019. Cell Death Dis. 1.036111111. PubMed
  65. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  66. Arcangeli S, et al. 2022. J Clin Invest. 132: . PubMed
  67. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  68. Mulder K, et al. 2021. Immunity. 54(8):1883-1900.e5. PubMed
  69. Osaka M, et al. 2016. Sci Rep. 6:21391. PubMed
  70. Davis M, et al. 2015. J Immunol. 194:2219. PubMed
  71. West D, et al. 2014. J Immunol. 192:1762. PubMed
  72. Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed
  73. Oldrini B, et al. 2018. Nat Commun. 9:1466. PubMed
  74. Xiong X et al. 2019. Mol Cell. 75(3):644-660 . PubMed
  75. She L, et al. 2019. J Immunol. 203:2520. PubMed
  76. Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed
  77. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  78. Wells KL, et al. 2020. Elife. 9:. PubMed
  79. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  80. Rangaraju S, et al. 2018. Mol Neurodegener. 13:24. PubMed
  81. Bogie JFJ, et al. 2021. J Autoimmun. 124:102723. PubMed
  82. Kovacs M, et al. 2021. Acta Neuropathol Commun. 9:136. PubMed
  83. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  84. Voss M, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L188 - L195. PubMed
  85. Peñaloza HF, et al. 2018. Biochem Biophys Rep. 13:12. PubMed
  86. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  87. Taddio MF, et al. 2021. Mol Imaging Biol. 23:196. PubMed
  88. Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed
  89. Wang X, et al. 2020. Nat Commun. 4.505555556. PubMed
  90. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  91. Zhang S, et al. 2022. Nat Commun. 13:4744. PubMed
  92. Li X, et al. 2021. Cell Death Dis. 12:314. PubMed
  93. Woods S, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:912. PubMed
  94. Enam S, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.07.013. PubMed
  95. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  96. Doni A, et al. 2021. Nat Commun. 12:3739. PubMed
  97. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  98. Gorina R, et al. 2014. J Immunol. 192:324. PubMed
  99. Kerkhofs D, et al. 2020. Theranostics. 7.022222222. PubMed
  100. Yamamuro T, et al. 2020. Nat Commun. 3.340277778. PubMed
  101. Gomzikova MO, et al. 2020. Pharmaceutics. 12:00. PubMed
  102. Yamasaki R, et al. 2014. J Exp Med. 211:1533. PubMed
  103. Westrich JA, et al. 2019. Oncogene. 38:7166. PubMed
  104. Liu Q, et al. 2021. Adv Mater. 33:e2102852. PubMed
  105. Sears SM, et al. 2022. Kidney360. 3:818. PubMed
  106. Wong L, et al. 2013. J Biol Chem. 288:35170. PubMed
  107. Yin X, et al. 2015. J Immunol. 194:4029. PubMed
  108. SL C, et al. 2016. JCI Insight. 1: 83116. PubMed
  109. Cao Z, et al. 2021. Molecular Cell. 81(17):3604-3622.e10. PubMed
  110. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  111. Bai C, et al. 2022. Exp Ther Med. 24:673. PubMed
  112. Chen R, et al. 2019. Mol Med Rep. 1.172916667. PubMed
  113. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  114. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
  115. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  116. Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed
  117. Chuang LT, et al. 2021. Molecules. 26:. PubMed
RRID
AB_893343 (BioLegend Cat. No. 103129)
AB_893339 (BioLegend Cat. No. 103130)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine5 anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS UV395™ anti-mouse CD45 30-F11 FC
Go To Top Version: 2    Revision Date: 05/14/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account