PerCP/Cyanine5.5 anti-mouse CD11a Antibody, CD11a, 2D7

Clone: 2D7 (See other available formats)
Regulatory Status: RUO
Other Names: αL integrin, LFA-1 α subunit, Ly-15, Ly-21, ITGAL
Isotype: Rat IgG2a, κ
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Product Citations: publications

2D7_PERCP-CYA55_CD11a_FC_122923 — C57BL/6 splenocytes were stained with anti-mouse CD11a (clone 2D7) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).

Compare all formats See PerCP/Cyanine5.5 spectral data

Cat #	Size	Price	Save
162907	25 µg	¥26,400
162908	100 µg	¥64,900

Description

CD11a is a 180 kD glycoprotein, also known as α_L integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3097463 (BioLegend Cat. No. 162907)
AB_3097463 (BioLegend Cat. No. 162908)

Antigen Details

Structure: Integrin, associated with integrin CD18, 180 kD
Distribution: Lymphocytes, granulocytes, monocytes, macrophages
Function: Cellular adhesion
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Cell Type: Granulocytes, Lymphocytes, Macrophages, Monocytes
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1. .Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479.
Gene ID: 16408 View all products for this Gene ID
UniProt: View information about CD11a on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD11a Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD11a Antibody	2D7	FC,IHC-F
Alexa Fluor® 488 anti-mouse CD11a	2D7	FC
APC/Fire™ 750 anti-mouse CD11a	2D7	FC
PerCP/Cyanine5.5 anti-mouse CD11a	2D7	FC
Brilliant Violet 421™ anti-mouse CD11a	2D7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12/29/2023

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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