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PerCP/Cyanine5.5 anti-mouse IL-9 Antibody

Pricing & Availability
Clone
RM9A4 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype
Rat IgG1, κ
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Product Citations
publications
RM9A4_PerCPCyanine55_IL-9_Antibody_1_041124
Enriched C57BL/6 mouse CD4+ T cells were stimulated with anti-mouse CD3 and CD28 in the presence of recombinant IL-4 and TGF-β for three days, then re-stimulated with PdBU and ionomycin (in the presence of brefeldin A) for 5 hours, fixed, permeabilized, and then stained with CD4 FITC and IL-9 (clone RM9A4) PerCP/Cyanine5.5 (left) or rat IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • RM9A4_PerCPCyanine55_IL-9_Antibody_1_041124
    Enriched C57BL/6 mouse CD4+ T cells were stimulated with anti-mouse CD3 and CD28 in the presence of recombinant IL-4 and TGF-β for three days, then re-stimulated with PdBU and ionomycin (in the presence of brefeldin A) for 5 hours, fixed, permeabilized, and then stained with CD4 FITC and IL-9 (clone RM9A4) PerCP/Cyanine5.5 (left) or rat IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
514111 25 µg ¥38,720
514112 100 µg ¥90,420
Description

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant full length mouse IL-9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

See the protocol for Th9 polarization of mouse splenocytes.

Application References

(PubMed link indicates BioLegend citation)
  1. Angkasekwinai P, et. al. 2010. Nat. Immunol. 11:250. (ICFC) PubMed
  2. Chang HC, et al. 2010. Nat. Immunol. 11:527. (ICFC) PubMed
  3. Takami M, et al. 2012. J Immunol. 188:4369. PubMed.
  4. Reynolds LA, et al. 2012. J. Immunol. 189:1113. PubMed.
Product Citations
  1. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  2. Garcia-Dominguez D, et al. 2022. Front Immunol. 13:948335. PubMed
RRID
AB_2562729 (BioLegend Cat. No. 514111)
AB_2562729 (BioLegend Cat. No. 514112)

Antigen Details

Structure
Cytokine; 30-40 kD (Mammalian)
Bioactivity
Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources
IL-2 activated TH2 lymphocytes
Cell Targets
T lymphocytes, erythroid precursors
Receptors
IL-9R
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V, et al. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y, et al. 1992. Leuk. Lymphoma 8:441.

Regulation
Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID
16198 View all products for this Gene ID
UniProt
View information about IL-9 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/22/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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