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Purified anti-ASC Antibody

Pricing & Availability
Clone
O93E9 (See other available formats)
Regulatory Status
RUO
Other Names
PYD and CARD domain-containing protein (PYCARD), Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain protein 5, Target of methylation-induced silencing (TMS1)
Isotype
Mouse IgG1, κ
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Product Citations
publications
093E9_Purified_ASC_Antibody_WB_043015
Total cell lysate of HL-60 (lane 1, 15 µg), HT-29 (lane 2, 15 µg), and mouse thymus (lane 3, 15 µg) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-ASC (clone O93E9) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • 093E9_Purified_ASC_Antibody_WB_043015
    Total cell lysate of HL-60 (lane 1, 15 µg), HT-29 (lane 2, 15 µg), and mouse thymus (lane 3, 15 µg) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-ASC (clone O93E9) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
Cat # Size Price Save
676502 100 µg ¥68,420
Description

Apoptosis-associated speck-like protein containing a CARD (ASC), also known as target of methylation-mediated silencing (TMS1), PYD, and CARD domain containing protein (PYCARD), is a 21.6 kD pro-apoptotic protein that contains a N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD). The TMS1 gene was originally found to be aberrantly methylated and silenced in various cancer cells. Expression of ASC can be induced by pro-apoptotic/inflammatory stimuli. In normal cells, this protein is localized to the cytoplasm. However, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. During apoptosis, ASC translocates from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis. The adaptor molecule ASC mediates AIM2-dependent caspase-1 activation. Also, the AIM2/ASC complex can act as a novel caspase-8 activation platform. Recent studes has shown that ASC reduces the phosphorylation of IKKα/β phosphorylation and inhibits NF-κB activity in primary melanoma. However, in metastatic melanoma through, it has the opposite function enhancing NF-κB activity and the secretion of IL-1β.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human PYCARD (NP_037390) produced in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Gritsenko A, et al. 2022. Eur J Immunol. 52:1141. PubMed
  2. Yan YQ, et al. 2022. Eur J Neurol. 29:3590. PubMed
  3. Santana DJ, et al. 2020. Curr Protoc Microbiol. 59:e124. PubMed
RRID
AB_2565643 (BioLegend Cat. No. 676502)

Antigen Details

Structure
Full-length ASC (fASC) contains 195 amino acids wtih a predicted molecular weight of 21.6 kD. The sequence of isoform ASC-b is missing 93-111 a.a. The sequence of isoform ASC-c is missing 26-85 a.a.
Distribution

Cytoplasm. In resting monocytes and macrophages, the nucleus is the primary localization.

Function
ASC promotes caspase-mediated apoptosis. This pro-apoptotic activity is mediated predominantly through the activation of caspase-9. ASC is an integral component of the inflammasome. Within the inflammasome, ASC links caspase-1 and NOD-like receptors (NLR), which lead to the activation of caspase-1.
Ligand/Receptor
CIAS1/PYPAF1 and PYDC1.
Cell Type
Dendritic cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Antigen References

1. Masumoto J, et al. 1999. J. Biol. Chem. 274:33835.
2. Conway KE, et al. 2000. Cancer Res. 60:6236.
3. Strong R, et al. 1991. Brain Res. 542:23.
4. Srinivasula SM, et al. 2002. J. Biol. Chem. 277:21119.
5. Ohtsuka T, et al. 2004. Nat. Cell Biol. 6:121.
6. Ippagunta SK, et al. 2010. J. Biol. Chem. 285:12454.
7. Liu W, et al. 2013. J. Invest Dermatol. 2:518-527.

Gene ID
29108 View all products for this Gene ID
UniProt
View information about ASC on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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