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Purified anti-CD3 Antibody (Previously Covance catalog# MMS-5212)

Pricing & Availability
Clone
BC3 (See other available formats)
Regulatory Status
RUO
Other Names
T-cell surface glycoprotein CD3 epsilon chain, CD3-epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T3E, TCRE
Previously
Covance Catalog# MMS-5212
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
BC3_Purified_CD3_Antibody_FC_022615
Human peripheral blood lymphocytes were stained with purified anti-CD3 antibody (clone BC3, green histogram) or purified mouse IgG2b, κ isotype control (orange histogram), followed by anti-mouse IgG PE.
  • BC3_Purified_CD3_Antibody_FC_022615
    Human peripheral blood lymphocytes were stained with purified anti-CD3 antibody (clone BC3, green histogram) or purified mouse IgG2b, κ isotype control (orange histogram), followed by anti-mouse IgG PE.
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Cat # Size Price Save
830301 100 µL ¥60,500
Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against human CD3.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 1.0 - 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in flow cytometry (FC).

RRID
AB_2564937 (BioLegend Cat. No. 830301)

Antigen Details

Biology Area
Cell Biology, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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