- Clone
- Poly19058 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Epidermal filaggrin
- Isotype
- Rabbit Polyclonal
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Reported Reactivity
- Non-Human Primate
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This monospecific polyclonal antibody was raised against a peptide corresponding to residues 24-39 in the partial sequence of filaggrin.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-F - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product may contain other non-IgG subtypes. -
Application References
(PubMed link indicates BioLegend citation) -
- Wallace L, et al. 2012. J. Cell Sci. 125:1750.
- Bayo P, et al. 2008. Endocrinology 149:1377.
- Hu Y, et al. 2001. Nature 410:710.
- Yuspa SH, et al. 1989. J. Cell Biol. 109:1207.
- Liang Y. 2011. Patholog. Res. Int. 2011:936794. (IHC) PubMed
- Roop DR, et al. 1984. J. Biol. Chem. 259:8037.
- Product Citations
-
- RRID
-
AB_2616957 (BioLegend Cat. No. 905804)
Antigen Details
- Structure
- Filaggrin is a 44 kD protein.
- Distribution
-
Cytoskeleton and nucleus.
- Function
- Mutations in this gene are associated with ichthyosis vulgaris.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure
- Gene ID
- 2312 View all products for this Gene ID
- UniProt
- View information about Filaggrin on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Filaggrin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Filaggrin | Poly19058 | IHC-F |
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Purified anti-Filaggrin
IHC staining of anti-Filaggrin antibody (Poly19058) on froze...
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