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- Clone
- W21036I (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were stained with purified anti-human CD275 (B7-H2, ICOSL) (clone W21036I) (left) or purified rat IgG2a, κ isotype control (right) followed by anti-rat IgG PE. The cells were stained with anti-human CD19 APC. Data shown were gated on lymphocyte population.
B7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by B lymphocytes, activated monocytes/macrophages, and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human B7-H2 recombinant protein (19-258 aa)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone W21036I completely blocks clone 9F.8A4 but does not block clone 2D3.
- RRID
-
AB_2941574 (BioLegend Cat. No. 387502)
Antigen Details
- Structure
- Ig superfamily, B7 family, 40 kD
- Distribution
-
B cells and dendritic cells
- Function
- Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production
- Interaction
- B cells, Dendritic cells
- Ligand/Receptor
- ICOS
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Wang S, et al. 2002. J Biol Chem. 96:2808.
- Wong SC, et al. 2003. Blood. 102:1831.
- Gene ID
- 23308 View all products for this Gene ID
- UniProt
- View information about CD275 on UniProt.org
Related FAQs
Other Formats
View All CD275 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD275 (B7-H2, ICOSL) | W21036I | FC |
| PE anti-human CD275 (B7-H2, ICOSL) | W21036I | FC |
| Ultra-LEAF™ Purified anti-human CD275 (B7-H2, ICOSL) | W21036I | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD275 (B7-H2, ICOSL)
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD275 (B7-H2, ICOSL)
Human peripheral blood lymphocytes were surface stained with... -
Ultra-LEAF™ Purified anti-human CD275 (B7-H2, ICOSL)
Biotinylated Recombinant Human B7-H2 (ICOSL)-Fc chimera (Cat...
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